2. Academic Validation
  3. Fluorogenic Probes for Multicolor Imaging in Living Cells

Fluorogenic Probes for Multicolor Imaging in Living Cells

  • J Am Chem Soc. 2016 Aug 3;138(30):9365-8. doi: 10.1021/jacs.6b04782.
Gražvydas Lukinavičius 1 2,  Luc Reymond 1,  Keitaro Umezawa 3,  Olivier Sallin 1,  Elisa D'Este 2,  Fabian Göttfert 2,  Haisen Ta 2,  Stefan W Hell 2,  Yasuteru Urano 3,  Kai Johnsson 1
Affiliations

Affiliations

  • 1 Institute of Bioengineering, NCCR in Chemical Biology, Institute of Chemical Sciences and Engineering (ISIC), Ecole Polytechnique Fédérale de Lausanne (EPFL) , 1015 Lausanne, Switzerland.
  • 2 Department of NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry , Am Fassberg 11, 37077 Göttingen, Germany.
  • 3 Graduate School of Pharmaceutical Sciences, The University of Tokyo , 7-3-1, Hongo, Bunkyo-ku, Tokyo 113-8654, Japan.
PMID: 27420907 DOI: 10.1021/jacs.6b04782
Abstract

Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes, and SNAP-tag are fluorogenic, cell-permeable, and compatible with superresolution microscopy. In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding our capacity for imaging living cells.

Figures
Products