2. Academic Validation
  3. SPATA2 promotes CYLD activity and regulates TNF-induced NF-κB signaling and cell death

SPATA2 promotes CYLD activity and regulates TNF-induced NF-κB signaling and cell death

  • EMBO Rep. 2016 Oct;17(10):1485-1497. doi: 10.15252/embr.201642592.
Lisa Schlicher 1,  Manuela Wissler 2,  Florian Preiss 3,  Prisca Brauns-Schubert 3,  Celia Jakob 2,  Veronica Dumit 4,  Christoph Borner 1,  Joern Dengjel 5,  Ulrich Maurer 6
Affiliations

Affiliations

  • 1 Institute of Molecular Medicine and Cell Research, Albert-Ludwigs-University Freiburg, Freiburg, Germany Spemann Graduate School of Biology and Medicine (SGBM), Albert-Ludwigs-University of Freiburg, Freiburg, Germany BIOSS, Centre for Biological Signaling Studies, Freiburg, Germany.
  • 2 Institute of Molecular Medicine and Cell Research, Albert-Ludwigs-University Freiburg, Freiburg, Germany.
  • 3 Institute of Molecular Medicine and Cell Research, Albert-Ludwigs-University Freiburg, Freiburg, Germany Spemann Graduate School of Biology and Medicine (SGBM), Albert-Ludwigs-University of Freiburg, Freiburg, Germany.
  • 4 Core Facility Proteomics, Center for Biological Systems Analysis, Freiburg, Germany.
  • 5 BIOSS, Centre for Biological Signaling Studies, Freiburg, Germany Core Facility Proteomics, Center for Biological Systems Analysis, Freiburg, Germany.
  • 6 Institute of Molecular Medicine and Cell Research, Albert-Ludwigs-University Freiburg, Freiburg, Germany Spemann Graduate School of Biology and Medicine (SGBM), Albert-Ludwigs-University of Freiburg, Freiburg, Germany BIOSS, Centre for Biological Signaling Studies, Freiburg, Germany [email protected].
PMID: 27458237 DOI: 10.15252/embr.201642592
Abstract

K63- and Met1-linked ubiquitylation are crucial posttranslational modifications for TNF Receptor signaling. These non-degradative ubiquitylations are counteracted by deubiquitinases (DUBs), such as the Enzyme CYLD, resulting in an appropriate signal strength, but the regulation of this process remains incompletely understood. Here, we describe an interaction partner of CYLD, SPATA2, which we identified by a mass spectrometry screen. We find that SPATA2 interacts via its PUB domain with CYLD, while a PUB interaction motif (Pim) of SPATA2 interacts with the PUB domain of the LUBAC component HOIP SPATA2 is required for the recruitment of CYLD to the TNF Receptor signaling complex upon TNFR stimulation. Moreover, SPATA2 acts as an allosteric activator for the K63- and M1-deubiquitinase activity of CYLD In consequence, SPATA2 substantially attenuates TNF-induced NF-κB and MAPK signaling. Conversely, SPATA2 is required for TNF-induced complex II formation, Caspase activation, and Apoptosis. Thus, this study identifies SPATA2 as an important factor in the TNF signaling pathway with a substantial role for the effects mediated by the cytokine.

Keywords

CYLD; HOIP; SPATA2; TNF; apoptosis.

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