2. Academic Validation
  3. A Long Noncoding RNA Regulates Sister Chromatid Cohesion

A Long Noncoding RNA Regulates Sister Chromatid Cohesion

  • Mol Cell. 2016 Aug 4;63(3):397-407. doi: 10.1016/j.molcel.2016.06.031.
Francesco P Marchese 1,  Elena Grossi 1,  Oskar Marín-Béjar 1,  Sanjay Kumar Bharti 2,  Ivan Raimondi 1,  Jovanna González 1,  Dannys Jorge Martínez-Herrera 1,  Alejandro Athie 1,  Alicia Amadoz 1,  Robert M Brosh Jr 2,  Maite Huarte 3
Affiliations

Affiliations

  • 1 Center for Applied Medical Research (CIMA), Department of Gene Therapy and Regulation of Gene Expression, University of Navarra, 55 Pio XII Avenue, 31008 Pamplona, Spain; Institute of Health Research of Navarra (IdiSNA), 31008 Pamplona, Spain.
  • 2 Laboratory of Molecular Gerontology, National Institute on Aging, National Institutes of Health, NIH Biomedical Research Center, 251 Bayview Boulevard, Baltimore, MD 21224, USA.
  • 3 Center for Applied Medical Research (CIMA), Department of Gene Therapy and Regulation of Gene Expression, University of Navarra, 55 Pio XII Avenue, 31008 Pamplona, Spain; Institute of Health Research of Navarra (IdiSNA), 31008 Pamplona, Spain. Electronic address: [email protected].
PMID: 27477908 DOI: 10.1016/j.molcel.2016.06.031
Abstract

Long noncoding RNAs (lncRNAs) are involved in diverse cellular processes through multiple mechanisms. Here, we describe a previously uncharacterized human lncRNA, CONCR (cohesion regulator noncoding RNA), that is transcriptionally activated by MYC and is upregulated in multiple Cancer types. The expression of CONCR is cell cycle regulated, and it is required for cell-cycle progression and DNA replication. Moreover, cells depleted of CONCR show severe defects in sister chromatid cohesion, suggesting an essential role for CONCR in cohesion establishment during cell division. CONCR interacts with and regulates the activity of DDX11, a DNA-dependent ATPase and helicase involved in DNA replication and sister chromatid cohesion. These findings unveil a direct role for an lncRNA in the establishment of sister chromatid cohesion by modulating DDX11 enzymatic activity.

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