MDA7/IL-24 is an anti-viral factor that inhibits influenza virus replication

J Microbiol. 2016 Oct;54(10):695-700. doi: 10.1007/s12275-016-6383-2.

Rak-Kyun Seong ¹, Young-Ki Choi ², Ok Sarah Shin ³

Affiliations

1 Brain Korea 21 Plus for Biomedical Science, College of Medicine, Korea University, Seoul, 08308, Republic of Korea.
2 College of Medicine and Medical Research Institute, Chungbuk National University, Cheongju, 28644, Republic of Korea.
3 Brain Korea 21 Plus for Biomedical Science, College of Medicine, Korea University, Seoul, 08308, Republic of Korea. [email protected].

PMID: 27687232 DOI: 10.1007/s12275-016-6383-2

Abstract

Melanoma differentiation associated gene-7 (mda-7)/interleukin- 24 (IL-24) is a secreted cytokine, which plays an essential role in tumor suppression. Although its role as a multifunctional protein affecting broad types of cancers is well described, functions of IL-24 in host defense against virus Infection are yet to be determined. In this study, we explored the anti-viral effect of recombinant IL-24 treatment during influenza Infection. Infection of human lung adenocarcinoma cells (A549) with the influenza A virus up-regulated IL-24 mRNA and protein expression in a time-dependent manner. Pre-treatment of A549 cells with recombinant IL-24 protein effectively suppressed viral plaque formation. Furthermore, IL-24 treatment of A549 cells reduced viral non-structural protein 1 (NS1) synthesis, whereas IL-24 knockdown resulted in increased viral replication. Interestingly, IL-24 treatment following influenza A virus Infection led to up-regulation of interferon (IFN)-induced Antiviral signaling. Taken together, our results suggest that IL-24 exerts a potent suppressive effect on influenza viral replication and can be used in the treatment of influenza Infection.

Keywords

IL-24; antiviral activities; influenza.

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