2. Academic Validation
  3. The tetrameric kinesin Kif25 suppresses pre-mitotic centrosome separation to establish proper spindle orientation

The tetrameric kinesin Kif25 suppresses pre-mitotic centrosome separation to establish proper spindle orientation

  • Nat Cell Biol. 2017 Apr;19(4):384-390. doi: 10.1038/ncb3486.
Justin Decarreau 1,  Michael Wagenbach 1,  Eric Lynch 2,  Aaron R Halpern 3,  Joshua C Vaughan 1 3,  Justin Kollman 2,  Linda Wordeman 1
Affiliations

Affiliations

  • 1 Department of Physiology and Biophysics, University of Washington, Seattle, Washington 98195, USA.
  • 2 Department of Biochemistry, University of Washington, Seattle, Washington 98195, USA.
  • 3 Department of Chemistry, University of Washington, Seattle, Washington 98195, USA.
PMID: 28263957 DOI: 10.1038/ncb3486
Abstract

Microtubules tether centrosomes together during interphase. How this is accomplished and what benefit it provides to the cell is not known. We have identified a bipolar, minus-end-directed Kinesin, Kif25, that suppresses centrosome separation. Kif25 is required to prevent premature centrosome separation during interphase. We show that premature centrosome separation leads to microtubule-dependent nuclear translocation, culminating in eccentric nuclear positioning that disrupts the cortical spindle positioning machinery. The activity of Kif25 during interphase is required to maintain a centred nucleus to ensure the spindle is stably oriented at the onset of mitosis.

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