2. Academic Validation
  3. Two-Color 810 nm STED Nanoscopy of Living Cells with Endogenous SNAP-Tagged Fusion Proteins

Two-Color 810 nm STED Nanoscopy of Living Cells with Endogenous SNAP-Tagged Fusion Proteins

  • ACS Chem Biol. 2018 Feb 16;13(2):475-480. doi: 10.1021/acschembio.7b00616.
Alexey N Butkevich 1,  Haisen Ta 1,  Michael Ratz 1,  Stefan Stoldt 1,  Stefan Jakobs 1 2,  Vladimir N Belov 1,  Stefan W Hell 1
Affiliations

Affiliations

  • 1 Department of NanoBiophotonics, Max-Planck Institute for Biophysical Chemistry , Am Fassberg 11, 37077 Göttingen, Germany.
  • 2 Department of Neurology, University of Göttingen Medical Faculty , Robert-Koch-Str. 40, 37075 Göttingen, Germany.
PMID: 28933823 DOI: 10.1021/acschembio.7b00616
Abstract

A 810 nm STED nanoscopy setup and an appropriate combination of two fluorescent dyes (Si-rhodamine 680SiR and carbopyronine 610CP) have been developed for near-IR live-cell super-resolution imaging. Vimentin endogenously tagged using the CRISPR/Cas9 approach with the SNAP tag, together with a noncovalent tubulin label, provided reliable and cell-to-cell reproducible dual-color confocal and STED imaging of the Cytoskeleton in living cells.

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