2. Academic Validation
  3. Development of a Positron Emission Tomography Radiotracer for Imaging Elevated Levels of Superoxide in Neuroinflammation

Development of a Positron Emission Tomography Radiotracer for Imaging Elevated Levels of Superoxide in Neuroinflammation

  • ACS Chem Neurosci. 2018 Mar 21;9(3):578-586. doi: 10.1021/acschemneuro.7b00385.
Catherine Hou 1,  Chia-Ju Hsieh 1,  Shihong Li 1,  Hsiaoju Lee 1,  Thomas J Graham 1,  Kuiying Xu 1,  Chi-Chang Weng 1,  Robert K Doot 1,  Wenhua Chu 2,  Subhasish K Chakraborty 3,  Laura L Dugan 3,  Mark A Mintun 2,  Robert H Mach 1
Affiliations

Affiliations

  • 1 Department of Radiology, Perelman School of Medicine , University of Pennsylvania , Philadelphia , Pennsylvania 19104 , United States.
  • 2 Mallinckrodt Institute of Radiology , Washington University School of Medicine , St. Louis , Missouri 63110-1016 , United States.
  • 3 Department of Medicine , Vanderbilt University School of Medicine , Nashville , Tennessee 37232 , United States.
PMID: 29099578 DOI: 10.1021/acschemneuro.7b00385
Abstract

Reactive Oxygen Species (ROS) are believed to play a major role in the proinflammatory, M1-polarized form of neuroinflammation. However, it has been difficult to assess the role of ROS and their role in neuroinflammation in animal models of disease because of the absence of probes capable of measuring their presence with the functional imaging technique positron emission tomography (PET). This study describes the synthesis and in vivo evaluation of [18F]ROStrace, a radiotracer for imaging superoxide in vivo with PET, in an LPS model of neuroinflammation. [18F]ROStrace was found to rapidly cross the blood-brain barrier (BBB) and was trapped in the brain of LPS-treated Animals but not the control group. [18F] ox-ROStrace, the oxidized form of [18F]ROStrace, did not cross the BBB. These data suggest that [18F]ROStrace is a suitable radiotracer for imaging superoxide levels in the central nervous system with PET.

Keywords

Neuroinflammation; Positron emission tomography; Reactive oxygen species; Superoxide.

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