2. Academic Validation
  3. CCDC102B functions in centrosome linker assembly and centrosome cohesion

CCDC102B functions in centrosome linker assembly and centrosome cohesion

  • J Cell Sci. 2018 Dec 3;131(23):jcs222901. doi: 10.1242/jcs.222901.
Yuqing Xia 1,  Ning Huang 1,  Zhiquan Chen 1,  Fangyuan Li 1,  Guiliang Fan 1,  Dandan Ma 1,  Jianguo Chen 2 3,  Junlin Teng 2
Affiliations

Affiliations

  • 1 Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, Beijing 100871, China.
  • 2 Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education and State Key Laboratory of Membrane Biology, College of Life Sciences, Peking University, Beijing 100871, China [email protected] [email protected].
  • 3 Center for Quantitative Biology, Peking University, Beijing 100871, China.
PMID: 30404835 DOI: 10.1242/jcs.222901
Abstract

The proteinaceous centrosome linker is an important structure that allows the centrosome to function as a single microtubule-organizing center (MTOC) in interphase cells. However, the assembly mechanism of the centrosome linker components remains largely unknown. In this study, we identify CCDC102B as a new centrosome linker protein that is required for maintaining centrosome cohesion. CCDC102B is recruited to the centrosome by C-Nap1 (also known as CEP250) and interacts with the centrosome linker components rootletin and LRRC45. CCDC102B decorates and facilitates the formation of rootletin filaments. Furthermore, CCDC102B is phosphorylated by Nek2A (an isoform encoded by NEK2) and is disassociated from the centrosome at the onset of mitosis. Together, our findings reveal a molecular role for CCDC102B in centrosome cohesion and centrosome linker assembly.This article has an associated First Person interview with the first authors of the paper.

Keywords

C-Nap1; CCDC102B; Centrosome cohesion; LRRC45; Nek2A; rootletin.

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