2. Academic Validation
  3. Induction of secondary metabolite production by hygromycin B and identification of the 1233A biosynthetic gene cluster with a self-resistance gene

Induction of secondary metabolite production by hygromycin B and identification of the 1233A biosynthetic gene cluster with a self-resistance gene

  • J Antibiot (Tokyo). 2020 Jul;73(7):475-479. doi: 10.1038/s41429-020-0295-4.
Sho Kato 1 2,  Takayuki Motoyama 3,  Masakazu Uramoto 1,  Toshihiko Nogawa 1,  Takashi Kamakura 4,  Hiroyuki Osada 5 6
Affiliations

Affiliations

  • 1 Chemical Biology Research Group, RIKEN CSRS, Wako, Saitama, Japan.
  • 2 Graduate School of Science and Engineering, Saitama University, Sakura, Saitama, Japan.
  • 3 Chemical Biology Research Group, RIKEN CSRS, Wako, Saitama, Japan. [email protected].
  • 4 Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, Noda, Chiba, Japan.
  • 5 Chemical Biology Research Group, RIKEN CSRS, Wako, Saitama, Japan. [email protected].
  • 6 Graduate School of Science and Engineering, Saitama University, Sakura, Saitama, Japan. [email protected].
PMID: 32139880 DOI: 10.1038/s41429-020-0295-4
Abstract

We found that the protein synthesis inhibitor hygromycin B induced the production of secondary metabolites, including lucilactaene, NG-391, fusarubin, 1233A, and 1233B, in the filamentous fungus, Fusarium sp. RK97-94. We identified the biosynthetic gene cluster for 1233A, an HMG-CoA synthase inhibitor. The biosynthetic gene cluster consisted of four genes, one of which was involved in conferring self-resistance to 1233A.

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