CCDC57 Cooperates with Microtubules and Microcephaly Protein CEP63 and Regulates Centriole Duplication and Mitotic Progression

Cell Rep. 2020 May 12;31(6):107630. doi: 10.1016/j.celrep.2020.107630.

H Kubra Gurkaslar ¹, Efraim Culfa ¹, Melis D Arslanhan ¹, Mariana Lince-Faria ², Elif Nur Firat-Karalar ³

Affiliations

1 Department of Molecular Biology and Genetics, Koç University, Sarıyer, İstanbul 34450, Turkey.
2 Instituto Gulbenkian de Ciência, Rua da Quinta Grande 6, Oeiras 2780-156, Portugal.
3 Department of Molecular Biology and Genetics, Koç University, Sarıyer, İstanbul 34450, Turkey. Electronic address: [email protected].

PMID: 32402286 DOI: 10.1016/j.celrep.2020.107630

Abstract

Centrosomes function in key cellular processes ranging from cell division to cellular signaling. Their dysfunction is linked to Cancer and developmental disorders. Here, we identify CCDC57 as a pleiotropic regulator of centriole duplication, mitosis, and ciliogenesis. Combining proximity mapping with superresolution imaging, we show that CCDC57 localizes to the proximal end of centrioles and interacts with the microcephaly protein CEP63, centriolar satellite proteins, and microtubules. Loss of CCDC57 causes defects in centriole duplication and results in a failure to localize CEP63 and CEP152 to the centrosome. Additionally, CCDC57 depletion perturbs mitotic progression both in wild-type and centriole-less cells. Importantly, its centrosome-targeting region is required for its interaction with CEP63 and functions during centriole duplication and cilium assembly, whereas the microtubule-targeting region is required for its mitotic functions. Together, our results identify CCDC57 as a critical interface between centrosome and microtubule-mediated cellular processes that are deregulated in microcephaly.

Keywords

BioID; CCDC57; CEP63; centriolar satellites; centriole duplication; centrosome; microtubules; mitosis; primary cilium; primary microcephaly.

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