2. Academic Validation
  3. [Progress in fluorescent visualization techniques for neurotransmitter detection]

[Progress in fluorescent visualization techniques for neurotransmitter detection]

  • Sheng Wu Gong Cheng Xue Bao. 2020 Jun 25;36(6):1051-1059. doi: 10.13345/j.cjb.190441.
Qiao Hu 1 2,  Yuxin Shi 1 2,  Xiaoling Yang 1 2,  Jing Zhang 1 2,  Bo Li 1 2,  Xiaoling Liao 1 2,  Xue Liu 1 2
Affiliations

Affiliations

  • 1 Chongqing Key Laboratory of Nano/Micro Composite Materials and Devices, Chongqing University of Science and Technology, Chongqing 401331, China.
  • 2 Chongqing Engineering Laboratory of Nano/Micro Biomedical Detection Technology, Chongqing University of Science and Technology, Chongqing 401331, China.
PMID: 32597056 DOI: 10.13345/j.cjb.190441
Abstract

Neurotransmitters play an important role in nervous system. Temporal and spatial changes of neurotransmitter distribution are crucial to information processing in neural networks. Biosensors that can visually monitor neurotransmitters are one of the vital tools to explore a variety of physiological and pathological activities. This article reviews recent advances in monitoring neurotransmitters with high temporal and spatial resolution, and introduces the latest fluorescent imaging methods for typical neurotransmitters, including glutamate, dopamine, γ-aminobutyric acid and acetylcholine. The article also summarizes the basic principles, advantages and disadvantages of various visually detection methods, and provides systematic suggestions for designing neurotransmitter sensors with high temporal and spatial resolution.

Keywords

fluorescence resonance energy transfer; fluorescent protein; neurotransmitter; visualization.

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