2. Academic Validation
  3. Novel spectrofluorimetric determination of brigatinib in bulk powder and human urine samples via ion-pair complex formation using eosin Y

Novel spectrofluorimetric determination of brigatinib in bulk powder and human urine samples via ion-pair complex formation using eosin Y

  • Spectrochim Acta A Mol Biomol Spectrosc. 2021 Mar 15;249:119210. doi: 10.1016/j.saa.2020.119210.
Halah S Almutairi 1,  Mona M AlShehri 1,  Mohammed M Alanazi 1,  Ibrahim A Darwish 1,  Hany W Darwish 2
Affiliations

Affiliations

  • 1 Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457 Riyadh, 11451, Saudi Arabia.
  • 2 Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457 Riyadh, 11451, Saudi Arabia; Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini St., Cairo 11562, Egypt. Electronic address: [email protected].
PMID: 33234480 DOI: 10.1016/j.saa.2020.119210
Abstract

The developed spectrofluorimetric method was successfully applied to the analysis of brigatinib (BRG) in its bulk powder form, and human urine sample. It is based on the investigation of the fluorescence spectrum behavior of the BRG-eosin Y complex. The relative fluorescence intensity (RFI) was recorded at 560 nm after excitation at 480 nm. The principle of the proposed method was thoroughly explained. All experimental parameters affecting method development were optimized. Moreover, the obtained results were fully discussed and statistically analyzed. The molar ratio method was applied to study the stoichiometric relationship between BRG and eosin Y complex. The method revealed a ratio of 1:3 for BRG-eosin Y afforded the highest RFI. The developed method was validated over the concentration range of 62.5-4000 ng mL-1. The results were compared positively with the reported method.

Keywords

Brigatinib; Eosin Y; Ion-pair complex; Spectrofluorimetry.

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