2. Academic Validation
  3. Effects of different concentrations of metformin on osteoclast differentiation and apoptosis and its mechanism

Effects of different concentrations of metformin on osteoclast differentiation and apoptosis and its mechanism

  • Pharmazie. 2021 Jun 1;76(6):244-248. doi: 10.1691/ph.2021.1378.
Feng Bian 1,  Yufeng Zhang 1,  YuanLong Xie 1,  Hongyu Fang 2,  Mingyu Fan 2,  Xin Wang 2,  Hongliang Li 2,  Tao Huang 2,  Sheng Zhang 1,  Lin Cai 3
Affiliations

Affiliations

  • 1 Department of Spine Surgery and Musculoskeletal Tumor, Zhongnan Hospital of Wuhan University, People's Republic of China.
  • 2 Department of Orthopedics, Wuhan Third Hospital, Wuhan City, People's Republic of China.
  • 3 Department of Spine Surgery and Musculoskeletal Tumor, Zhongnan Hospital of Wuhan University, People's Republic of China;, Email: [email protected].
PMID: 34078517 DOI: 10.1691/ph.2021.1378
Abstract

This study aimed to investigate the effect of metformin on osteoclast differentiation and Apoptosis. Low concentration of metformin inhibited osteoclast differentiation and downregulated the expression of TRAP, RANK, Cathepsink, NFATC-1, MMP-9 and TRAF-6. High concentration of metformin promoted osteoclast Apoptosis and upregulated the expression of Bax/Bcl-2 and caspase-3; BV/TV, BS/TV, Tb.N and BMD were increased while Tp.Sp decreased in the group of intraperitoneal metformin+femoral intramedullary osteoclast injection (Met+OC) compared with the control group, 1 nM metformin downregulated Akt, p44/42 MAPK, JNK, p38 MAPK phosphorylation, 5 nM metformin down regulated ERK and Akt phosphorylation. These results suggest that a low concentration of metformin inhibits osteoclast differentiation through PI3K/Akt and MAPK/ERK signaling pathway; high concentrations of metformin promote osteoclast Apoptosis through PI3K/Akt and ERK signaling pathway.

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