2. Academic Validation
  3. Diterpenoids from Euphorbia helioscopia and their enhancement of NK cell-mediated killing of NSCLC cells

Diterpenoids from Euphorbia helioscopia and their enhancement of NK cell-mediated killing of NSCLC cells

  • Bioorg Chem. 2022 Feb;119:105534. doi: 10.1016/j.bioorg.2021.105534.
Hong-Yan Mu 1,  Chen-Yuan Gong 2,  Ru-Jun Zhang 3,  Zhen-Hua Chen 3,  Shi-Guo Zhu 4,  Wei-Min Zhao 5
Affiliations

Affiliations

  • 1 Natural Product Research Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
  • 2 Center for Traditional Chinese Medicine and Immunology Research School of Basic Medical Sciences, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China; Department of Immunology and Pathogenic Biology, School of Basic Medical Sciences, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China.
  • 3 Natural Product Research Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China.
  • 4 Center for Traditional Chinese Medicine and Immunology Research School of Basic Medical Sciences, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China; Department of Immunology and Pathogenic Biology, School of Basic Medical Sciences, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People's Republic of China. Electronic address: [email protected].
  • 5 Natural Product Research Center, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, People's Republic of China; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China. Electronic address: [email protected].
PMID: 34894576 DOI: 10.1016/j.bioorg.2021.105534
Abstract

Fourteen previously undescribed Diterpenoids, including an unusual diterpenoid (1) with a 9,10-seco-jatrophane skeleton, ten jatrophane-type Diterpenoids (2-11), two lathyrane-type Diterpenoids (12, 13), and an abietane-type diterpenoid (14), together with thirty-six known ones (15-50), were isolated from the whole Plants of Euphorbia helioscopia L. The structures of the new isolates were characterized by spectroscopic methods, single-crystal X-ray diffraction analysis, and computational prediction of ECD and chemical shifts. Thirty-nine abundant Diterpenoids were evaluated for their enhancement of NK cell-mediated killing of NSCLC cells. As a result, compounds 24, 33, and 41 were found to significantly enhance the killing activity of NK cells towards H1299-luci cells and A549-luci cells at the concentration of 2.5 μM.

Keywords

Diterpenoids; Euphorbia helioscopia; NK cell-mediated killing; NSCLC cells.

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