2. Academic Validation
  3. Applications of Tandem Mass Spectrometry (MS/MS) in Protein Analysis for Biomedical Research

Applications of Tandem Mass Spectrometry (MS/MS) in Protein Analysis for Biomedical Research

  • Molecules. 2022 Apr 8;27(8):2411. doi: 10.3390/molecules27082411.
Anca-Narcisa Neagu 1,  Madhuri Jayathirtha 2,  Emma Baxter 2,  Mary Donnelly 2,  Brindusa Alina Petre 2 3,  Costel C Darie 2
Affiliations

Affiliations

  • 1 Laboratory of Animal Histology, Faculty of Biology, "Alexandru Ioan Cuza" University of Iasi, Carol I bvd. No. 22, 700505 Iasi, Romania.
  • 2 Biochemistry & Proteomics Group, Department of Chemistry and Biomolecular Science, Clarkson University, 8 Clarkson Avenue, Potsdam, NY 13699, USA.
  • 3 Laboratory of Biochemistry, Faculty of Chemistry, "Alexandru Ioan Cuza" University of Iasi, Carol I bvd. No. 22A, 700505 Iasi, Romania.
PMID: 35458608 DOI: 10.3390/molecules27082411
Abstract

Mass Spectrometry (MS) allows the analysis of proteins and Peptides through a variety of methods, such as Electrospray Ionization-Mass Spectrometry (ESI-MS) or Matrix-Assisted Laser Desorption Ionization-Mass Spectrometry (MALDI-MS). These methods allow identification of the mass of a protein or a peptide as intact molecules or the identification of a protein through peptide-mass fingerprinting generated upon enzymatic digestion. Tandem mass spectrometry (MS/MS) allows the fragmentation of proteins and Peptides to determine the amino acid sequence of proteins (top-down and middle-down proteomics) and Peptides (bottom-up proteomics). Furthermore, tandem mass spectrometry also allows the identification of post-translational modifications (PTMs) of proteins and Peptides. Here, we discuss the application of MS/MS in biomedical research, indicating specific examples for the identification of proteins or Peptides and their PTMs as relevant biomarkers for diagnostic and therapy.

Keywords

biomarkers; biomedical research; proteomics; tandem mass spectrometry (MS/MS).

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