2. Academic Validation
  3. Bioelectronic cell-based device provides a strategy for the treatment of the experimental model of multiple sclerosis

Bioelectronic cell-based device provides a strategy for the treatment of the experimental model of multiple sclerosis

  • J Control Release. 2022 Nov 16;352:994-1008. doi: 10.1016/j.jconrel.2022.11.008.
Emilie Audouard 1,  Fanny Michel 2,  Vanessa Pierroz 2,  Taeuk Kim 2,  Lisa Rousselot 1,  Béatrix Gillet-Legrand 1,  Gaëlle Dufayet-Chauffaut 1,  Peter Buchmann 2,  Michael Florea 2,  Alexander Khel 3,  Kamilya Altynbekova 3,  Claudia Delgaldo 4,  Encarna Escudero 4,  Alejandra Ben Aissa Soler 4,  Nathalie Cartier 1,  Francoise Piguet 1,  Marc Folcher 5
Affiliations

Affiliations

  • 1 NeuroGenCell, Paris Brain Institute - ICM, INSERM, CNRS, AP-HP, Sorbonne Université; Hôpital de la Pitié Salpêtrière, Paris, France.
  • 2 Department of Biosystems Science and Engineering, D-BSSE, ETH Zürich, Basel, Switzerland.
  • 3 GeneXplain GmbH, Wolfenbuettel, Germany.
  • 4 Eurecat, Centre Tecnològic de Catalunya, Functional Printing and Embedded Devices Unit, Mataró, Spain.
  • 5 Department of Biosystems Science and Engineering, D-BSSE, ETH Zürich, Basel, Switzerland; Institute of Molecular and Clinical Ophthalmology, IOB, Basel, Switzerland. Electronic address: [email protected].
PMID: 36370877 DOI: 10.1016/j.jconrel.2022.11.008
Abstract

Wireless powered optogenetic cell-based implant provides a strategy to deliver subcutaneously therapeutic proteins. Immortalize Human Mesenchymal Stem Cells (hMSC-TERT) expressing the bacteriophytochrome diguanylate cyclase (DGCL) were validated for optogenetic controlled interferon-β delivery (Optoferon cells) in a bioelectronic cell-based implant. Optoferon cells transcriptomic profiling was used to elaborate an in-silico model of the recombinant interferon-β production. Wireless optoelectronic device integration was developed using additive manufacturing and injection molding. Implant cell-based optoelectronic interface manufacturing was established to integrate industrial flexible compact low-resistance screen-printed Near Field Communication (NFC) coil antenna. Optogenetic cell-based implant biocompatibility, and device performances were evaluated in the Experimental Autoimmune Encephalomyelitis (EAE) mouse model of multiple sclerosis.

Keywords

Bioelectronic; Cell-based device; Drug delivery; Interferon; Multiple sclerosis; Optogenetic.

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