2. Academic Validation
  3. Rapid extraction of osimertinib and its active metabolite in urine by miniaturized centrifugal spin-column extraction using ionic liquid hybrid hierarchical porous adsorbent

Rapid extraction of osimertinib and its active metabolite in urine by miniaturized centrifugal spin-column extraction using ionic liquid hybrid hierarchical porous adsorbent

  • J Chromatogr A. 2023 Jul 20;1705:464224. doi: 10.1016/j.chroma.2023.464224.
Yehong Han 1,  Mingyu Wang 2,  Chunliu Yang 3,  Zihui Zhao 4,  Yanan Yuan 4,  Hongyuan Yan 5
Affiliations

Affiliations

  • 1 State Key Laboratory of New Pharmaceutical Preparations and Excipients, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, School of Life Science, College of Pharmaceutical Science, Hebei University, Baoding 071002, China; Hebei Key Laboratory of Public Health Safety, College of Public Health, Hebei University, Baoding 071002, China.
  • 2 Department of Pharmacy, Affiliated Hospital of Hebei University, Baoding 071002, China. Electronic address: [email protected].
  • 3 Hebei Key Laboratory of Public Health Safety, College of Public Health, Hebei University, Baoding 071002, China.
  • 4 State Key Laboratory of New Pharmaceutical Preparations and Excipients, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, School of Life Science, College of Pharmaceutical Science, Hebei University, Baoding 071002, China.
  • 5 State Key Laboratory of New Pharmaceutical Preparations and Excipients, Key Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of Education, School of Life Science, College of Pharmaceutical Science, Hebei University, Baoding 071002, China; Hebei Key Laboratory of Public Health Safety, College of Public Health, Hebei University, Baoding 071002, China. Electronic address: [email protected].
PMID: 37490816 DOI: 10.1016/j.chroma.2023.464224
Abstract

Osimertinib (OSIM) is widely used as a mainstream drug for the treatment of non-small cell lung Cancer (NSCLC). However, the lack of a rapid extraction and detection method for OSIM and its metabolite, AZ-5104, has limited clinical drug metabolism and drug resistance research because the drug is unstable. In this study, a new ionic liquid hybrid hierarchical porous material (IL-HHPM) was synthesized with hierarchical porous structures, including micropores (1.6-2.0 nm), mesopores (2.0-50.0 nm), macropores (50.0-148.7 nm), and multiple functional groups via a one-step hydrothermal method using silanized ionic liquids (IL) as functionalized hybrid monomer. The IL-HHPM has the advantages of a high specific surface area (437.4 ± 4.6 m2 g-1), sizable pore volume (0.74 cm3 g-1), and fast mass transfer, additionally, the IL-HHPM adsorbed OSIM and AZ-5104 via π-π interactions and hydrogen bonding. OSIM and AZ-5104 were rapidly extracted and measured in human urine using rapid and miniaturized centrifugal spin-column extraction (MCSCE), which was based on the IL-HHPM. The optimized factors for the extraction recoveries of OSIM and AZ-5104 were adsorbent dosage (8.0 mg), sample volume (0.5 mL), and operation time (9.0 min), and markedly reduced the adsorbent dosage and operation time. The IL-HHPM-MCSCE-HPLC method displayed good linearity (0.02-5.00 μg mL-1, r ≥ 0.9997), satisfying accuracy (spiked recoveries of 87.7%-100.0%), and good precision (RSDs ≤ 7.0%). The developed method is rapid, sensitive, and reproducible for the simultaneous determination of trace level of OSIM and AZ-5104 in human urine.

Keywords

AZ-5104; Ionic liquid hybrid hierarchical porous adsorbent; Liquid Chromatography; Miniaturized centrifugal spin-column extraction; Osimertinib.

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