NSD2-IN-1
Based on 1 Customer Validation
NSD2-IN-1 (compound 38) is a potent and high selective NSD2-PWWP1 (nuclear receptor-binding SET domain 2-PWWP1) inhibitor, with an IC50 of 0.11 μM. NSD2-IN-1 can bind to NSD2-PWWP1 and then affect the expression of genes regulated by NSD2. NSD2-IN-1 induces apoptosis and cell cycle arrest.
For research use only. We do not sell to patients.
- Purity: 98.72%
- CAS No.: 2797183-37-8
- Formula: C29H31N5
- Molecular Weight:449.59
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Apoptosis Compound Library
HY-L003
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Anti-Cancer Compound Library
HY-L025
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Nuclear Receptor Compound Library
HY-L126
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Heterocyclic Compound Library
HY-L138
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Highly Selective Activators Library
HY-L159
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Cell Death Library
HY-L162
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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Non-Alcoholic Fatty Liver Disease (NAFLD) Compound Library
HY-L199
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High-Throughput Bioactive Compound Library
HY-L205
Biological Activity
IC50: 0.11 ± 0.01 μM (NSD2)[1]
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| KMS-11 | IC50 |
8.43 μM
Compound: 38
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Antiproliferative activity against human KMS-11 cells assessed as inhibition of cell proliferation measured after 6 days by CCK8 assay
Antiproliferative activity against human KMS-11 cells assessed as inhibition of cell proliferation measured after 6 days by CCK8 assay
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[PMID: 35704853] |
| KMS-11 | IC50 |
8.43 μM
Compound: 15
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Antiproliferative activity against human KMS-11 cells assessed as inhibition of cell growth
Antiproliferative activity against human KMS-11 cells assessed as inhibition of cell growth
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[PMID: 37578463] |
| MM1.S | IC50 |
10.95 μM
Compound: 38
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Antiproliferative activity against human MM1.S cells assessed as inhibition of cell proliferation measured after 6 days by CCK8 assay
Antiproliferative activity against human MM1.S cells assessed as inhibition of cell proliferation measured after 6 days by CCK8 assay
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[PMID: 35704853] |
| MM1.S | IC50 |
10.95 μM
Compound: 15
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Antiproliferative activity against human MM1.S cells assessed as inhibition of cell growth
Antiproliferative activity against human MM1.S cells assessed as inhibition of cell growth
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[PMID: 37578463] |
| MV4-11 | IC50 |
2.23 μM
Compound: 38
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Antiproliferative activity against human MV4-11 cells assessed as inhibition of cell proliferation measured after 6 days by CCK8 assay
Antiproliferative activity against human MV4-11 cells assessed as inhibition of cell proliferation measured after 6 days by CCK8 assay
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[PMID: 35704853] |
| MV4-11 | IC50 |
2.23 μM
Compound: 15
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Antiproliferative activity against human MV4-11 cells
Antiproliferative activity against human MV4-11 cells
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[PMID: 37578463] |
| RS4-11 | IC50 |
6.3 μM
Compound: 38
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Antiproliferative activity against human RS4-11 cells assessed as inhibition of cell proliferation measured after 6 days by CCK8 assay
Antiproliferative activity against human RS4-11 cells assessed as inhibition of cell proliferation measured after 6 days by CCK8 assay
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[PMID: 35704853] |
NSD2-IN-1 inhibits cell proliferation in MV4:11, RS4:11, KMS11, and MM1S cells, with IC50 values of 2.23, 6.30, 8.43, and 10.95 μM, respectively[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2797183-37-8
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Appearance Solid
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Molecular Weight 449.59
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Formula C29H31N5
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Color Off-white to light yellow
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SMILES
CN1C=NC(C2=C(C=C(C=C2C)CN3CCC(CC3)N)C)=C1C4=CC=C(C5=C4C=CC=C5)C#N
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 50 mg/mL (111.21 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.56 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (5.56 mM); Clear solution
This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (270 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.2242 mL | 11.1212 mL | 22.2425 mL | 55.6062 mL |
| 5 mM | 0.4448 mL | 2.2242 mL | 4.4485 mL | 11.1212 mL | |
| 10 mM | 0.2224 mL | 1.1121 mL | 2.2242 mL | 5.5606 mL | |
| 15 mM | 0.1483 mL | 0.7414 mL | 1.4828 mL | 3.7071 mL | |
| 20 mM | 0.1112 mL | 0.5561 mL | 1.1121 mL | 2.7803 mL | |
| 25 mM | 0.0890 mL | 0.4448 mL | 0.8897 mL | 2.2242 mL | |
| 30 mM | 0.0741 mL | 0.3707 mL | 0.7414 mL | 1.8535 mL | |
| 40 mM | 0.0556 mL | 0.2780 mL | 0.5561 mL | 1.3902 mL | |
| 50 mM | 0.0445 mL | 0.2224 mL | 0.4448 mL | 1.1121 mL | |
| 60 mM | 0.0371 mL | 0.1854 mL | 0.3707 mL | 0.9268 mL | |
| 80 mM | 0.0278 mL | 0.1390 mL | 0.2780 mL | 0.6951 mL | |
| 100 mM | 0.0222 mL | 0.1112 mL | 0.2224 mL | 0.5561 mL |
NSD2-IN-1 Related Classifications
HY-147914 Related Classifications
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