Dual-luciferase assay (Luciferase, LUC) is a biotechnology widely used in the study of gene expression regulation and cell signaling pathways. This experiment uses luciferase genes to be transfected into cells and the expression level of the target gene is quantified by detecting the luciferase activity produced. Luciferase experiments are sensitive, high-throughput, and can be applied to in vivo imaging and high-throughput screening. This technology provides powerful tools in the fields of gene regulation, protein interaction, drug screening and other fields, and promotes biomedical research and drug development.