The pyroptosis assay is an experimental method used to evaluate cells undergoing pyroptosis, a specific form of cell death. Typically, this involves using techniques such as fluorescent probes, cell morphology observation, or Western blot to detect characteristics of pyroptosis, such as cell membrane rupture, DNA fragmentation, and changes in the expression of specific proteins. Pyroptosis is a controlled form of cell death that regulates inflammation and immune responses. Studying pyroptosis can help to gain a deeper understanding of its role in physiological and pathological processes and related disease mechanisms.