56 KDA type-specific antigen Protein, Orientia tsutsugamushi (Cell-Free, His)

56 KDA type‐specific antigenic proteins have dual roles as key adhesion factors for adsorption of rickettsiae to host cell surfaces and as determinants of the virulence of individual rickettsial strains. As a major outer membrane protein, it occupies a central position in the interaction between pathogens and host cells. 56 KDA type-specific antigen Protein, Orientia tsutsugamushi (Cell-Free, His) is the recombinant 56 KDA type-specific antigen protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of 56 KDA type-specific antigen Protein, Orientia tsutsugamushi (Cell-Free, His) is 499 a.a., with molecular weight of 57 kDa.

The 56 KDA type-specific antigen Protein appears to serve dual roles as a crucial adherent factor for rickettsial adsorption to the host-cell surface and a determinant of virulence in individual rickettsial strains. Its significance lies in being the major outer membrane protein, suggesting a central position in the interaction between the pathogen and host cells. As an adherent factor, the protein likely plays a pivotal role in the initial stages of infection by facilitating the binding of rickettsial particles to host cells. Additionally, its role in determining the virulence of specific rickettsial strains underscores its potential impact on the pathogenicity and infectious outcomes associated with these bacterial agents. Elucidating the precise mechanisms by which the 56 KDA type-specific antigen contributes to rickettsial adherence and virulence could enhance our understanding of host-pathogen interactions and provide insights for developing targeted therapeutic strategies.

Others

E. coli Cell-free

N-10*His

P37917 (I23-F521)

/

IEFDENSLECGPYAKVGIVGGVLSGVESARLDPADSEGKKHLPLIKGMPFGVTLAAGMTITPGVRAEISAMYLMNVKAEVELGKMGSDANTGTTADASAGVIRKHKKLTPPQPNIMPISIADRDIAVDIPNAAGQGNVDVRAAARIAWLKNYAGIDYYVPDSNNPQGRVVNPVLLNIPQGNPNPAGGGGRAAPAAFDILDHAQWRDVVVGITALSNANKPNVSAVKILSDKISQIYADIKPFANVASVQISETPLPDSASVDQIQNKVQELNKVLEDVRESFDGFILNAFAQPVRLNFQIPQVVQGQGQQPQAAATAQEAAAAAAIRALNDGENNGIIQLYKDLYKLQRNVALKKSMKQLGDELGVDQGQEGGCSKDKKQSDTTAEESKKEGKKGKEIEFDLHMAVGQVKLYADLFTIDSFSVYAGIGAGLAYTHGKIDGKDIKAHTGMVGSLALGVAANVADGVYMDVDAGYLYSFSKIEEKYQMNAFVASAGIRYNF

57 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.