ABCD2 Protein, Human (Cell-Free, His)

ABCD2 protein is an ATP-dependent transporter in the ABC family that promotes the transport of very long chain fatty acids (VLCFA)-CoA into peroxisomes and exhibits fatty acyl-CoA thioesterase (ACOT) and ATPase activities. Its substrate specificity overlaps with ABCD1, including a clear preference for shorter VLCFA (C22:0) and polyunsaturated fatty acids (PUFA) such as C22:6-CoA and C24:6-CoA. ABCD2 Protein, Human (Cell-Free, His) is the recombinant human-derived ABCD2 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of ABCD2 Protein, Human (Cell-Free, His) is 740 a.a., with molecular weight of 89.3 kDa.

As an ATP-dependent transporter belonging to the ATP-binding cassette (ABC) family, ABCD2 protein is integral to the transport of very long chain fatty acid (VLCFA)-CoA from the cytosol to the peroxisome lumen. Recent studies suggest that, akin to ABCD1, ABCD2 exhibits fatty acyl-CoA thioesterase (ACOT) and ATPase activities. According to this model, VLCFA-CoA is transported in an ATP-dependent manner into peroxisomes following the hydrolysis of VLCFA-CoA, mediated by the ACOT activity of ABCD2 (Probable). The substrate specificity of ABCD2 overlaps with ABCD1, particularly toward saturated fatty acids (FA) and monounsaturated FA (MUFA), but it demonstrates a distinct preference for shorter VLCFA (C22:0) and polyunsaturated fatty acids (PUFA) such as C22:6-CoA and C24:6-CoA in vitro. This suggests a potential role for ABCD2 in the regulation of very long chain fatty acids (VLCFAs) and energy metabolism, particularly in the degradation and biosynthesis of fatty acids through beta-oxidation.

Human

E. coli Cell-free

N-10*His

Q9UBJ2 (M1-S740)

225

MTHMLNAAADRVKWTRSSAAKRAACLVAAAYALKTLYPIIGKRLKQSGHGKKKAAAYPAAENTEILHCTETICEKPSPGVNADFFKQLLELRKILFPKLVTTETGWLCLHSVALISRTFLSIYVAGLDGKIVKSIVEKKPRTFIIKLIKWLMIAIPATFVNSAIRYLECKLALAFRTRLVDHAYETYFTNQTYYKVINMDGRLANPDQSLTEDIMMFSQSVAHLYSNLTKPILDVMLTSYTLIQTATSRGASPIGPTLLAGLVVYATAKVLKACSPKFGKLVAEEAHRKGYLRYVHSRIIANVEEIAFYRGHKVEMKQLQKSYKALADQMNLILSKRLWYIMIEQFLMKYVWSSSGLIMVAIPIITATGFADGEDGQKQVMVSERTEAFTTARNLLASGADAIERIMSSYKEVTELAGYTARVYNMFWVFDEVKRGIYKRTAVIQESESHSKNGAKVELPLSDTLAIKGKVIDVDHGIICENVPIITPAGEVVASRLNFKVEEGMHLLITGPNGCGKSSLFRILSGLWPVYEGVLYKPPPQHMFYIPQRPYMSLGSLRDQVIYPDSVDDMHDKGYTDQDLERILHNVHLYHIVQREGGWDAVMDWKDVLSGGEKQRMGMARMFYHKPKYALLDECTSAVSIDVEGKIFQAAKGAGISLLSITHRPSLWKYHTHLLQFDGEGGWRFEQLDTAIRLTLSEEKQKLESQLAGIPKMQQRLNELCKILGEDSVLKTIKNEDETS

89.3 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.