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Basigin/CD147 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

Basigin/BSG proteins are critical for retinal maturation and development and function as retinal cell receptors for NXNL1, promoting the survival of retinal cone photoreceptors. Basigin/CD147 Protein, Human (HEK293, His) is the recombinant human-derived Basigin/CD147 protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Basigin/CD147 Protein, Human (HEK293, His)
  • Experimental Validation Results for Basigin/CD147 Protein, Human (HEK293, His)
  • Basigin/CD147 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

Basigin/BSG proteins are critical for retinal maturation and development and function as retinal cell receptors for NXNL1, promoting the survival of retinal cone photoreceptors. Basigin/CD147 Protein, Human (HEK293, His) is the recombinant human-derived Basigin/CD147 protein, expressed by HEK293 , with C-6*His labeled tag.

Background

Basigin/BSG protein is essential for the normal maturation and development of the retina, functioning as a critical cell surface receptor for NXNL1 and playing a pivotal role in NXNL1-mediated survival of retinal cone photoreceptors. Collaborating with glucose transporter SLC16A1/GLUT1 and NXNL1, Basigin/BSG promotes the survival of retinal cones by facilitating aerobic glycolysis and accelerating glucose entry into photoreceptors. Additionally, it serves as a potent inducer of IL6 secretion in various cell lines, including monocytes. In the context of microbial infection, Basigin/BSG acts as an erythrocyte receptor for P. falciparum RH5, playing an indispensable role in erythrocyte invasion by the merozoite stage of P. falciparum isolates 3D7 and Dd2. These diverse functions highlight the multifaceted roles of Basigin/BSG in cellular processes and host-pathogen interactions.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized SARS-COV-2 S RBD (HY-P7431) at 1 μg/mL (100 μL/well) on the plate can bind Biotinylated Human CD147. The ED50 for this effect is 6.982 μg/mL.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    P35613-2 (A22-H205)

  • Gene ID
    682  [NCBI]
  • Molecular Construction
    • N-term
    • Basigin (A22-H205)
      Accession # P35613-2
    • 6*His
    • C-term
  • Protein Length

    Partial Extracellular Domain

  • Synonyms

    BSG; Leukocyte Activation Antigen M6; Prev. OK; Hepatoma-Associated Antigen; EMMPRIN; OK Blood Group Antigen; Basigin; HAb18G; Tumor Cell-Derived Collagenase Stimulatory Factor; TCSF; Extracellular Matrix Metalloproteinase Inducer; 5F7; EMPRIN; Ok Blood G

  • AA Sequence

    AAGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQKTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWYKITDSEDKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSH

  • Predicted Molecular Mass

    21.2 kDa

  • Molecular Weight

    Approximately 26-40 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized SARS-COV-2 S RBD (HY-P7431) at 1 μg/mL (100 μL/well) on the plate can bind biotinylated Human CD147. The ED50 for this effect is 6.982 μg/mL.

  • Basigin/CD147 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Basigin/CD147 Protein, Human (HEK293, His)
  • Experimental Validation Results for Basigin/CD147 Protein, Human (HEK293, His)
  • Basigin/CD147 Protein, Human (HEK293, His) Product Image