CACNA1C Protein, Pig (Cell-Free, His)
The CACNA1C protein is the alpha-1C subunit of voltage-gated calcium channels that generate L-type calcium currents that are critical for calcium influx and sarcoplasmic release. Its role in excitation-contraction coupling is critical for cardiac development, rhythm regulation, and smooth muscle cell contraction. CACNA1C Protein, Pig (Cell-Free, His) is the recombinant pig-derived CACNA1C protein, expressed by E. coli Cell-free , with N-10*His labeled tag.
- Species: Pig
- Source: E. coli Cell-free
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The CACNA1C protein is the alpha-1C subunit of voltage-gated calcium channels that generate L-type calcium currents that are critical for calcium influx and sarcoplasmic release. Its role in excitation-contraction coupling is critical for cardiac development, rhythm regulation, and smooth muscle cell contraction. CACNA1C Protein, Pig (Cell-Free, His) is the recombinant pig-derived CACNA1C protein, expressed by E. coli Cell-free , with N-10*His labeled tag.
Background
CACNA1C, the pore-forming alpha-1C subunit of the voltage-gated calcium channel, gives rise to L-type calcium currents, mediating the influx of calcium ions into the cytoplasm and triggering calcium release from the sarcoplasm. With a crucial role in excitation-contraction coupling in the heart, CACNA1C is essential for normal heart development, heart rhythm regulation, and the contraction of smooth muscle cells in blood vessels and the intestine. It plays a pivotal role in the regulation of blood pressure by contributing to the contraction of arterial smooth muscle cells. As a member of the 'high-voltage activated' (HVA) group, CACNA1C's long-lasting calcium channels are inhibited by dihydropyridines like isradipine and nifedipine. The channel activity is intricately regulated by Ca(2+) and calmodulin, with binding of STAC1, STAC2, or STAC3 inhibiting channel inactivation by Ca(2+) and calmodulin. Moreover, shear stress and pressure increase calcium channel activity, adding another layer of regulation to its dynamic functionality.
Technical Parameters
-
Species Pig
-
Source E. coli Cell-free
-
Tag N-10*His
-
Accession
O35505 (F1-Y169)
-
Gene ID100135490
-
Molecular Construction
-
N-term
-
10*His
-
CACNA1C (F1-Y169)
Accession # O35505 -
C-term
-
-
Protein Length
Partial
-
Synonyms
CACNA1C; CACNA1C Intronic Transcript 2; Prev. CACNL1A1; Voltage-Gated L-Type Calcium Channel Cav1.2 Alpha 1 Subunit, Splice Variant 9*; Prev. CCHL1A1; Calcium Channel, L Type, Alpha-1 Polypeptide, Isoform 1, Cardiac Muscle; Prev. CACNA1C-IT2; Calcium Chan
-
AA Sequence
FQEQGEQEYKNCELDKNQRQCVEYALKARPLRRYIPISITFFRLFRVMRLVKLLSRGEGIRTLLWTFIKSFQALPYVALLIVMLFFIYAVIGMQVFGKIALNDTTEINRNNNFQTFPQAVLLLFRCATGEAWQDIMLACMPGKKRAPESEPSNSTEGETPCGSSFAVFY
-
Predicted Molecular Mass
22.3 kDa
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
