CLCN3 Protein, Human (Cell-Free, His)

CLCN3 protein, a member of the CLC channel family, acts as an outwardly rectifying, electrogenic H(+)/Cl(-) exchanger. It facilitates the exchange of chloride ions for protons, reflecting its role in dynamic ion exchange. The conserved gating glutamate residues in the CLC family suggest their functionality as antiporters, emphasizing CLCN3's robust outward rectification and electrogenic nature in chloride and proton exchange. CLCN3 Protein, Human (Cell-Free, His) is the recombinant human-derived CLCN3 protein, expressed by E. coli Cell-free , with C-6*His labeled tag. The total length of CLCN3 Protein, Human (Cell-Free, His) is 818 a.a., with molecular weight of 93.2 kDa.

CLCN3 protein functions as a strongly outwardly rectifying, electrogenic H(+)/Cl(-) exchanger, facilitating the exchange of chloride ions against protons. As a member of the CLC channel family, which encompasses both chloride channels and proton-coupled anion transporters, CLCN3 is involved in the exchange of chloride or another anion for protons. The family's characteristic presence of conserved gating glutamate residues suggests its members' functionality as antiporters. The robust outward rectification and electrogenic nature of CLCN3 underscore its role in the dynamic exchange between chloride ions and protons.

Human

E. coli Cell-free

C-6*His

P51790 (M1-N818)

1182

MASMTGGQQMGRDPMESEQLFHRGYYRNSYNSITSASSDEELLDGAGVIMDFQTSEDDNLLDGDTAVGTHYTMTNGGSINSSTHLLDLLDEPIPGVGTYDDFHTIDWVREKCKDRERHRRINSKKKESAWEMTKSLYDAWSGWLVVTLTGLASGALAGLIDIAADWMTDLKEGICLSALWYNHEQCCWGSNETTFEERDKCPQWKTWAELIIGQAEGPGSYIMNYIMYIFWALSFAFLAVSLVKVFAPYACGSGIPEIKTILSGFIIRGYLGKWTLMIKTITLVLAVASGLSLGKEGPLVHVACCCGNIFSYLFPKYSTNEAKKREVLSAASAAGVSVAFGAPIGGVLFSLEEVSYYFPLKTLWRSFFAALVAAFVLRSINPFGNSRLVLFYVEYHTPWYLFELFPFILLGVFGGLWGAFFIRANIAWCRRRKSTKFGKYPVLEVIIVAAITAVIAFPNPYTRLNTSELIKELFTDCGPLESSSLCDYRNDMNASKIVDDIPDRPAGIGVYSAIWQLCLALIFKIIMTVFTFGIKVPSGLFIPSMAIGAIAGRIVGIAVEQLAYYHHDWFIFKEWCEVGADCITPGLYAMVGAAACLGGVTRMTVSLVVIVFELTGGLEYIVPLMAAVMTSKWVGDAFGREGIYEAHIRLNGYPFLDAKEEFTHTTLAADVMRPRRNDPPLAVLTQDNMTVDDIENMINETSYNGFPVIMSKESQRLVGFALRRDLTIAIESARKKQEGIVGSSRVCFAQHTPSLPAESPRPLKLRSILDMSPFTVTDHTPMEIVVDIFRKLGLRQCLVTHNGRLLGIITKKDILRHMAQTANQDPASIMFN

93.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.