CNGA4 Protein, Human (Cell-Free, His, SUMO)

The CNGA4 protein is a regulatory subunit of cation-selective cyclic nucleotide-gated (CNG) channels in olfactory sensory neurons that responds to cAMP to induce depolarization. This critical role in odorant signal transduction and adaptation enhances neural responses to olfactory stimulation. CNGA4 Protein, Human (Cell-Free, His, SUMO) is the recombinant human-derived CNGA4 protein, expressed by E. coli Cell-free , with N-6*His, N-SUMO labeled tag. The total length of CNGA4 Protein, Human (Cell-Free, His, SUMO) is 575 a.a., with molecular weight of 82 kDa.

CNGA4 protein serves as the modulatory subunit of cation-selective cyclic nucleotide-gated (CNG) channels, responding to the second messenger cAMP and inducing depolarization in olfactory sensory neurons (OSNs). These channels play a pivotal role in transducing odorant signals and subsequent adaptation, contributing to the neural response to various olfactory stimuli. CNGA4 facilitates rapid adaptation to odor stimulation by accelerating calcium-mediated negative feedback in olfactory signaling, thereby expanding the range of odor detection. The inhibitory effect of calcium-calmodulin on cAMP sensitivity involves its binding to the IQ-like motif of CNGA4, with a preference for the closed state of the channel. Additionally, the suppression of the CNG channel by PIP3 likely occurs through CGNA2 binding, highlighting the intricate regulatory mechanisms governing olfactory signal transduction.

Human

E. coli Cell-free

N-6*His;N-SUMO

Q8IV77 (M1-E575)

1262

MSQDTKVKTTESSPPAPSKARKLLPVLDPSGDYYYWWLNTMVFPVMYNLIILVCRACFPDLQHGYLVAWLVLDYTSDLLYLLDMVVRFHTGFLEQGILVVDKGRISSRYVRTWSFFLDLASLMPTDVVYVRLGPHTPTLRLNRFLRAPRLFEAFDRTETRTAYPNAFRIAKLMLYIFVVIHWNSCLYFALSRYLGFGRDAWVYPDPAQPGFERLRRQYLYSFYFSTLILTTVGDTPPPAREEEYLFMVGDFLLAVMGFATIMGSMSSVIYNMNTADAAFYPDHALVKKYMKLQHVNRKLERRVIDWYQHLQINKKMTNEVAILQHLPERLRAEVAVSVHLSTLSRVQIFQNCEASLLEELVLKLQPQTYSPGEYVCRKGDIGQEMYIIREGQLAVVADDGITQYAVLGAGLYFGEISIINIKGNMSGNRRTANIKSLGYSDLFCLSKEDLREVLSEYPQAQTIMEEKGREILLKMNKLDVNAEAAEIALQEATESRLRGLDQQLDDLQTKFARLLAELESSALKIAYRIERLEWQTREWPMPEDLAEADDEGEPEEGTSKDEEGRASQEGPPGPE

82 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.