CSEN Protein, Human (His)

CSEN, a calcium-dependent transcriptional repressor, binds to the DRE element of genes including PDYN and FOS. Its affinity for DNA is influenced by calcium, with reduced binding in the presence of calcium and enhanced binding when bound to magnesium. CSEN is implicated in nociception, suggesting a potential role in the regulation of pain sensation. Additionally, it serves as a regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, specifically interacting with channels such as KCND2/Kv4.2 and KCND3/Kv4.3. CSEN modulates these channels' expression at the cell membrane, influencing gating characteristics, inactivation kinetics, and the rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. The diverse functions of CSEN highlight its involvement in both transcriptional regulation and the modulation of ion channel activity, suggesting a multifaceted role in cellular processes. CSEN Protein, Human (His) is the recombinant human-derived CSEN protein, expressed by E. coli , with N-His labeled tag. The total length of CSEN Protein, Human (His) is 256 a.a., with molecular weight of ~29 kDa.

CSEN, a calcium-dependent transcriptional repressor, binds to the DRE element of genes including PDYN and FOS. Its affinity for DNA is influenced by calcium, with reduced binding in the presence of calcium and enhanced binding when bound to magnesium. CSEN is implicated in nociception, suggesting a potential role in the regulation of pain sensation. Additionally, it serves as a regulatory subunit of Kv4/D (Shal)-type voltage-gated rapidly inactivating A-type potassium channels, specifically interacting with channels such as KCND2/Kv4.2 and KCND3/Kv4.3. CSEN modulates these channels' expression at the cell membrane, influencing gating characteristics, inactivation kinetics, and the rate of recovery from inactivation in a calcium-dependent and isoform-specific manner. The diverse functions of CSEN highlight its involvement in both transcriptional regulation and the modulation of ion channel activity, suggesting a multifaceted role in cellular processes.

Human

E. coli

N-His

Q9Y2W7 (M1-I256)

30818  [NCBI]

MQPAKEVTKASDGSLLGDLGHTPLSKKEGIKWQRPRLSRQALMRCCLVKWILSSTAPQGSDSSDSELELSTVRHQPEGLDQLQAQTKFTKKELQSLYRGFKNECPTGLVDEDTFKLIYAQFFPQGDATTYAHFLFNAFDADGNGAIHFEDFVVGLSILLRGTVHEKLKWAFNLYDINKDGYITKEEMLAIMKSIYDMMGRHTYPILREDAPAEHVERFFEKMDRNQDGVVTIEEFLEACQKDENIMSSMQLFENVI

Approximately 29 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 8.0, 10% Glycerol.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.