DGKE Protein, Human (Cell-Free, His, SUMO, Myc)

DGKE protein is a membrane-bound diacylglycerol kinase that balances cell signaling by converting diacylglycerol (DAG) to phosphatidic acid (PA). As a central switch in signaling pathways, DGKE has opposite effects on various biological processes and significantly promotes complex lipid biosynthesis. DGKE Protein, Human (Cell-Free, His, SUMO, Myc) is the recombinant human-derived DGKE protein, expressed by E. coli Cell-free , with N-10*His, C-Myc, N-SUMO labeled tag. The total length of DGKE Protein, Human (Cell-Free, His, SUMO, Myc) is 567 a.a., with molecular weight of 83.9 kDa.

DGKE Protein, a membrane-bound diacylglycerol kinase, plays a crucial role in cellular signaling by converting diacylglycerol (DAG) into phosphatidic acid (PA), thereby orchestrating the balance between these bioactive lipids. Serving as a central switch in signaling pathways activated by these second messengers, DGKE exerts opposing effects on various biological processes. Additionally, DGKE contributes significantly to the biosynthesis of complex lipids. Notably, it exhibits specificity for diacylglycerol substrates with an arachidonoyl acyl chain at the sn-2 position, with a preference for specific molecular species within the phosphatidylinositol turnover cycle. While it can phosphorylate diacylglycerol substrates with a linoleoyl acyl chain at the sn-2 position, its efficiency in this process is comparatively lower. These functions highlight the intricate regulatory role of DGKE in cellular lipid signaling and metabolism.

Human

E. coli Cell-free

N-10*His;C-Myc;N-SUMO

P52429 (M1-E567)

8526

MEAERRPAPGSPSEGLFADGHLILWTLCSVLLPVFITFWCSLQRSRRQLHRRDIFRKSKHGWRDTDLFSQPTYCCVCAQHILQGAFCDCCGLRVDEGCLRKADKRFQCKEIMLKNDTKVLDAMPHHWIRGNVPLCSYCMVCKQQCGCQPKLCDYRCIWCQKTVHDECMKNSLKNEKCDFGEFKNLIIPPSYLTSINQMRKDKKTDYEVLASKLGKQWTPLIILANSRSGTNMGEGLLGEFRILLNPVQVFDVTKTPPIKALQLCTLLPYYSARVLVCGGDGTVGWVLDAVDDMKIKGQEKYIPQVAVLPLGTGNDLSNTLGWGTGYAGEIPVAQVLRNVMEADGIKLDRWKVQVTNKGYYNLRKPKEFTMNNYFSVGPDALMALNFHAHREKAPSLFSSRILNKAVYLFYGTKDCLVQECKDLNKKVELELDGERVALPSLEGIIVLNIGYWGGGCRLWEGMGDETYPLARHDDGLLEVVGVYGSFHCAQIQVKLANPFRIGQAHTVRLILKCSMMPMQVDGEPWAQGPCTVTITHKTHAMMLYFSGEQTDDDISSTSDQEDIKATE

83.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.