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Enolase 1/ENO1 Protein, Rat (His)

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Based on 1 Customer Validation

Enolase 1 (ENO1) plays a key role in carbohydrate degradation, particularly in the glycolytic pathway, where it catalyzes the conversion of 2-phospho-D-glycerate to phosphoenolpyruvate.This enzyme activity occurs during the fourth step of glycolysis and marks a critical stage in the conversion of glucose to pyruvate.Enolase 1/ENO1 Protein, Rat (His) is the recombinant rat-derived Enolase 1/ENO1 protein, expressed by E.coli , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Rat
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for Enolase 1/ENO1 Protein, Rat (His)
  • Enolase 1/ENO1 Protein, Rat (His) Product Image
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

Enolase 1 (ENO1) plays a key role in carbohydrate degradation, particularly in the glycolytic pathway, where it catalyzes the conversion of 2-phospho-D-glycerate to phosphoenolpyruvate.This enzyme activity occurs during the fourth step of glycolysis and marks a critical stage in the conversion of glucose to pyruvate.Enolase 1/ENO1 Protein, Rat (His) is the recombinant rat-derived Enolase 1/ENO1 protein, expressed by E.coli , with N-His labeled tag.

Background

Enolase 1 (ENO1) is a key enzyme involved in carbohydrate degradation, specifically within the glycolytic pathway, where it catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. This reaction occurs in the penultimate steps of glycolysis and is crucial for the generation of pyruvate from D-glyceraldehyde 3-phosphate. ENO1 plays a central role in cellular energy metabolism by participating in the glycolytic process, converting intermediates into pyruvate, which can further enter various metabolic pathways. The enzymatic activity of ENO1 contributes to the production of ATP, making it an essential component of energy production in cells. Serval researches indicateENO1's involvement in the specific biochemical transformation from D-glyceraldehyde 3-phosphate to pyruvate during glycolysis, emphasizing its significance in cellular energy metabolism.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Rat
  • Source E. coli
  • Tag N-His
  • Accession

    Q5BJ93 (M1-K434)

  • Gene ID
    24333  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • ENO1 (M1-K434)
      Accession # Q5BJ93
    • C-term
  • Protein Length

    Full Length

  • Synonyms

    ENO1; Enolase 1, (Alpha); Prev. ENO1L1; NNE; Prev. MPB1; Epididymis Secretory Protein Li 17; Prev. ENO1-IT1; C-Myc Promoter-Binding Protein-1; 2-Phospho-D-Glycerate Hydro-Lyase; MYC Promoter-Binding Protein 1; Phosphopyruvate Hydratase; C-Myc Promoter-Binding Protein; Alpha-Enolase; Alpha Enolase Like 1; MBP-1; Tau-Crystallin; PPH; Enolase-Alpha; ENO1 Intronic Transcript 1 (Non-Protein Coding); HEL-S-17; Plasminogen-Binding Protein; MPB-1; ENO1 Intronic Transcript 1; MBPB1; Enolase 1; Non-Neural Enolase

  • AA Sequence

    MSILKIHAREIFDSRGNPTVEVDLYTAKGLFRAAVPSGASTGIYEALELRDNDKTRFMGKGVSKAVEHINKTIAPALVSKKLNVVEQEKIDQLMIEMDGTENKSKFGANAILGVSLAVCKAGAVEKGVPLYRHIADLAGNPEVILPVPAFNVINGGSHAGNKLAMQEFMILPVGASSFREAMRIGAEVYHNLKNVIKEKYGKDATNVGDEGGFAPNILENKEALELLKSAIAKAGYTDQVVIGMDVAASEFYRAGKYDLDFKSPDDASRYITPDQLADLYKSFIKDYPVVSIEDPFDQDDWDAWQKFTATAGIQVVGDDLTVTNPKRIAKAAGEKSCNCLLLKVNQIGSVTESLQACKLAQSNGWGVMVSHRSGETEDTFIADLVVGLCTGQIKTGAPCRSERLAKYNQILRIEEELGSKAKFAGRSFRNPLAK

  • Predicted Molecular Mass

    49.4 kDa

  • Molecular Weight

    50 kDa

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 10% glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • Enolase 1/ENO1 Protein, Rat (His) Product Image
    1/16
  • Experimental Validation Results for Enolase 1/ENO1 Protein, Rat (His)
  • Enolase 1/ENO1 Protein, Rat (His) Product Image