Enolase 1/ENO1 Protein, Rat (His)

Enolase 1 (ENO1) plays a pivotal role in carbohydrate degradation, specifically in the glycolytic pathway, where it catalyzes the conversion of 2-phospho-D-glycerate to phosphoenolpyruvate. This enzymatic activity occurs during the fourth step of glycolysis, marking a crucial stage in the conversion of glucose to pyruvate. As a key player in glycolysis, ENO1 contributes to the energy production process by facilitating the generation of pyruvate, a central metabolite in cellular metabolism. This step underscores the essential function of ENO1 in maintaining energy homeostasis and supporting various cellular processes reliant on glycolytic intermediates. Enolase 1/ENO1 Protein, Rat (His) is the recombinant rat-derived Enolase 1/ENO1 protein, expressed by E. coli , with N-His labeled tag. The total length of Enolase 1/ENO1 Protein, Rat (His) is 434 a.a., with molecular weight of ~49.4 kDa.

Enolase 1 (ENO1) plays a pivotal role in carbohydrate degradation, specifically in the glycolytic pathway, where it catalyzes the conversion of 2-phospho-D-glycerate to phosphoenolpyruvate. This enzymatic activity occurs during the fourth step of glycolysis, marking a crucial stage in the conversion of glucose to pyruvate. As a key player in glycolysis, ENO1 contributes to the energy production process by facilitating the generation of pyruvate, a central metabolite in cellular metabolism. This step underscores the essential function of ENO1 in maintaining energy homeostasis and supporting various cellular processes reliant on glycolytic intermediates.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Rat

E. coli

N-His

Q5BJ93 (M1-K434)

24333  [NCBI]

Approximately 49.4 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 10% Glycerol, pH 8.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.