eptA Protein, E.coli strain K12 (Cell-Free, His)

The eptA protein plays a crucial role in bacterial resistance to polymyxins by catalyzing the addition of a phosphoethanolamine moiety to lipid A. This modification is essential for conferring resistance to the antimicrobial effects of polymyxins. eptA Protein, E.coli strain K12 (Cell-Free, His) is the recombinant E. coli-derived eptA protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of eptA Protein, E.coli strain K12 (Cell-Free, His) is 547 a.a., with molecular weight of 64.5 kDa.

EptA is an enzyme that catalyzes the addition of a phosphoethanolamine moiety to lipid A, a component of lipopolysaccharides (LPS) in bacterial cell membranes. This modification is crucial for conferring resistance to polymyxin, an antibiotic that targets the bacterial outer membrane. The phosphoethanolamine addition to lipid A modifies its structure, contributing to bacterial defense mechanisms against polymyxin-induced membrane disruption. EptA has been identified as part of a complex, with one known component being the ZipA-EptA fusion, along with an unidentified 24 kDa protein. This suggests that EptA's function is intricately linked to other proteins in the complex, possibly forming a coordinated system involved in bacterial membrane integrity and resistance to antimicrobial agents.

E.coli

E. coli Cell-free

N-10*His

P30845 (M1-E547)

75169632

MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDSLHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDRSMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGANILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVRIGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTASCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACDRVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNRYPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSLVYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQTQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE

64.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.