FCRN Protein, Mouse (HEK293, His)

The FCRN protein is a cell surface receptor that conveys passive humoral immunity through the selective uptake of monomeric IgG in milk. FCRN Protein, Mouse (HEK293, His) is a recombinant protein dimer complex containing mouse-derived FCRN protein, expressed by HEK293 , with C-6*His labeled tag. FCRN Protein, Mouse (HEK293, His), has molecular weight of 42-58 & 12 kDa, respectively.

FCRN, a vital cell surface receptor, facilitates the transfer of passive humoral immunity from the mother to the newborn. Recognizing the Fc region of monomeric immunoglobulin gamma, it selectively uptakes IgG from milk, particularly at the apical surface of the intestinal epithelium. The resultant FcRn-IgG complexes undergo transcytosis across the intestinal epithelium, releasing IgG from FcRn into blood or tissue fluids. This process contributes significantly to effective humoral immunity by recycling IgG and extending its half-life in the circulation. Mechanistically, monomeric IgG binding to FcRn in acidic endosomes of endothelial and hematopoietic cells facilitates the recycling of IgG to the cell surface, releasing it into circulation. Notably, besides its role in IgG homeostasis, the FcRn complex, consisting of two subunits, p51, and p14 (equivalent to beta-2-microglobulin), forms an MHC class I-like heterodimer, highlighting its pivotal role in immune and protein homeostasis. Furthermore, FCRN interacts with albumin/ALB, regulating the homeostasis of this other most abundant circulating protein.

Mouse

HEK293

C-6*His

Q61559 (Ser22-Val301)&P01887 (Ile21-Met119)

14132  [NCBI]&12010  [NCBI]

SETRPPLMYHLTAVSNPSTGLPSFWATGWLGPQQYLTYNSLRQEADPCGAWMWENQVSWYWEKETTDLKSKEQLFLEALKTLEKILNGTYTLQGLLGCELASDNSSVPTAVFALNGEEFMKFNPRIGNWTGEWPETEIVANLWMKQPDAARKESEFLLNSCPERLLGHLERGRRNLEWKEPPSMRLKARPGNSGSSVLTCAAFSFYPPELKFRFLRNGLASGSGNCSTGPNGDGSFHAWSLLEVKRGDEHHYQCQVEHEGLAQPLTVDLDSSARSSVPVV
&:
IQKTPQIQVYSRHPPENGKPNILNCYVTQFHPPHIEIQMLKNGKKIPKVEMSDMSFSKDWSFYILAHTEFTPTETDTYACRVKHASMAEPKTVYWDRDM

42-58&12 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.