FKTN Protein, Human (Cell-Free, His)

The FKTN protein catalyzes the transfer of ribitol phosphate from CDP-ribitol to the distal N-acetylgalactosamine in the phosphorylated O-mannosyl trisaccharide of α-dystroglycan (DAG1). FKTN Protein, Human (Cell-Free, His) is the recombinant human-derived FKTN protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of FKTN Protein, Human (Cell-Free, His) is 461 a.a., with molecular weight of 56.5 kDa.

FKTN protein plays a crucial role in catalyzing the transfer of a ribitol-phosphate from CDP-ribitol to the distal N-acetylgalactosamine of the phosphorylated O-mannosyl trisaccharide found in alpha-dystroglycan (DAG1). This enzymatic activity represents the initial step in the formation of the ribitol 5-phosphate tandem repeat, linking the phosphorylated O-mannosyl trisaccharide to the ligand binding moiety composed of repeats of 3-xylosyl-alpha-1,3-glucuronic acid-beta-1. FKTN is essential for maintaining the normal localization of POMGNT1 in Golgi membranes and ensuring its proper activity. Additionally, FKTN may participate in interacting with and reinforcing a large complex that encompasses both the external and internal muscle membranes. There is also a potential association with brain development.

Human

E. coli Cell-free

N-10*His

O75072 (M1-Y461)

2218

MSRINKNVVLALLTLTSSAFLLFQLYYYKHYLSTKNGAGLSKSKGSRIGFDSTQWRAVKKFIMLTSNQNVPVFLIDPLILELINKNFEQVKNTSHGSTSQCKFFCVPRDFTAFALQYHLWKNEEGWFRIAENMGFQCLKIESKDPRLDGIDSLSGTEIPLHYICKLATHAIHLVVFHERSGNYLWHGHLRLKEHIDRKFVPFRKLQFGRYPGAFDRPELQQVTVDGLEVLIPKDPMHFVEEVPHSRFIECRYKEARAFFQQYLDDNTVEAVAFRKSAKELLQLAAKTLNKLGVPFWLSSGTCLGWYRQCNIIPYSKDVDLGIFIQDYKSDIILAFQDAGLPLKHKFGKVEDSLELSFQGKDDVKLDVFFFYEETDHMWNGGTQAKTGKKFKYLFPKFTLCWTEFVDMKVHVPCETLEYIEANYGKTWKIPVKTWDWKRSPPNVQPNGIWPISEWDEVIQLY

56.5 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.