GULO Protein, Rat (Cell-Free, His)

GULO Protein facilitates the oxidation of L-gulono-1,4-lactone, generating hydrogen peroxide and L-xylo-hexulonolactone. The latter undergoes spontaneous isomerization, leading to the production of L-ascorbate, commonly known as vitamin C. GULO's enzymatic role in this pathway underscores its significance in the cellular synthesis of L-ascorbate, a crucial antioxidant supporting redox balance and essential physiological functions. GULO Protein, Rat (Cell-Free, His) is the recombinant rat-derived GULO protein, expressed by E. coli Cell-free , with C-10*His labeled tag. The total length of GULO Protein, Rat (Cell-Free, His) is 439 a.a., with molecular weight of 52.0 kDa.

GULO Protein catalyzes the oxidation of L-gulono-1,4-lactone, resulting in the production of hydrogen peroxide and L-xylo-hexulonolactone. The latter compound spontaneously undergoes isomerization, ultimately yielding L-ascorbate. This enzymatic process highlights the pivotal role of GULO in the biosynthesis of L-ascorbate, commonly known as vitamin C, a crucial antioxidant in various biological systems. The ability of GULO to facilitate this oxidation pathway contributes to the cellular synthesis of L-ascorbate, emphasizing its significance in maintaining redox balance and supporting essential physiological functions.

Rat

E. coli Cell-free

C-10*His

P10867 (V2-Y440)

60671

VHGYKGVQFQNWAKTYGCSPEVYYQPTSVEEVREVLALAREQKKKVKVVGGGHSPSDIACTDGFMIHMGKMNRVLQVDKEKKQITVEAGILLADLHPQLDEHGLAMSNLGAVSDVTVAGVIGSGTHNTGIKHGILATQVVALTLMTADGEVLECSESRNADVFQAARVHLGCLGIILTVTLQCVPQFHLQETSFPSTLKEVLDNLDSHLKRSEYFRFLWFPHTENVSIIYQDHTNKAPSSASNWFWDYAIGFYLLEFLLWTSTYLPCLVGWINRFFFWMLFNCKKESSNLSHKIFTYECRFKQHVQDWAIPREKTKEALLELKAMLEAHPKVVAHYPVEVRFTRGDDILLSPCFQRDSCYMNIIMYRPYGKDVPRLDYWLAYETIMKKFGGRPHWAKAHNCTQKDFEEMYPTFHKFCDIREKLDPTGMFLNSYLEKVFY

52.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.