HCoV-HKU1 Hemagglutinin esterase Protein, HCoV-HKU1 (Cell-Free, His)

The HCoV-HKU1 hemagglutinin esterase has a short spike and is critical for viral attachment and initiation of infection. This multifunctional protein exhibits receptor binding and destructive activity, specifically mediating de-O-acetylation of possible viral receptor determinants. HCoV-HKU1 Hemagglutinin esterase Protein, HCoV-HKU1 (Cell-Free, His) is the recombinant Virus-derived HCoV-HKU1 Hemagglutinin esterase protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of HCoV-HKU1 Hemagglutinin esterase Protein, HCoV-HKU1 (Cell-Free, His) is 374 a.a., with molecular weight of 45.9 kDa.

HCoV-HKU1 Hemagglutinin esterase is a structural protein characterized by short spikes on the virus surface, playing a crucial role in viral attachment and infection initiation. This multifunctional protein exhibits both receptor binding and receptor-destroying activities, specifically mediating the de-O-acetylation of N-acetyl-4-O-acetylneuraminic acid, a probable receptor determinant recognized by the virus on erythrocytes and susceptible cells. The receptor-destroying activity is vital for facilitating virus release, preventing self-aggregation, and ensuring the efficient spread of the progeny virus between cells. Serving as a secondary viral attachment protein, complementing the primary spike protein, it may become a target for immune responses, both humoral and cellular, contributing to the host defense against viral infections.

Virus

E. coli Cell-free

N-10*His

Q14EB1 (Y12-G385)

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YGFNEPLNVVSHLNHDWFLFGDSRSDCNHINNLKIKNYGYLDIHPSLCNNGKISSSAGDSIFKSYHFTRFYNYTGEGDQIIFYEGVNFNPHHRFKCFFNGSNDVWIFNKVRFYRALYSNMALFRYLTFVDILYNFSFSIKANICNSNILSLNNPIFISTNYSKDVYFTLSGCSLYLVPLCLFKSNFSQYYYNMDTGFAYGYSNFVSSDLDCTYISLKPGSYKIFSTGFVLSIPTKALCFNKSKQFVPVQVVDSRWNNLRASDTSLSDACQLPYCYFRNSSGNYVGKYDINHGDNGFTSILSGLLYNVSCISYYGSFLYDNFTSIWPRFSFGNCPTSAYIKLNCFYDPLPIILQGILLFLALLFIVFLLFLVYHG

45.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.