Hemagglutinin neuraminidase/HN Protein, Newcastle disease virus (Cell-Free, His)

The Hemagglutinin Neuraminidase (HN) protein facilitates viral entry by attaching the virus to cell receptors and inducing fusion with host membranes. It works with the fusion (F) protein and enables the spread of the virus by dissociating mature virions from glycoproteins. HN's crucial role in infection and propagation makes it essential for viral success. Hemagglutinin neuraminidase/HN Protein, Newcastle disease virus (Cell-Free, His) is the recombinant Virus-derived Hemagglutinin neuraminidase/HN protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of Hemagglutinin neuraminidase/HN Protein, Newcastle disease virus (Cell-Free, His) is 571 a.a., with molecular weight of 68.7 kDa.

The Hemagglutinin Neuraminidase (HN) protein plays a crucial role in the viral entry process by mediating the entry of the virus into the host cell, working in coordination with the fusion (F) protein. HN attaches the virus to sialic acid-containing cell receptors, initiating the infection process. Upon binding to the receptor, the HN protein induces a conformational change that enables the F protein to trigger the fusion of virion and cell membranes. Additionally, the neuraminidase activity of HN is instrumental in facilitating the efficient spread of the virus. This activity is responsible for dissociating mature virions from neuraminic acid-containing glycoproteins, contributing to the overall success of viral propagation.

Virus

E. coli Cell-free

N-10*His

P35741 (M1-V571)

/

MDRAVSRVALENEEREAKNTWRFVFRIAILLLIVITLAISAAALVYSMEASTPGDLVGIPTVISRAEEKITSALSSNQDVVDRIYKQVALESPLALLNTESVIMNAITSLSYQINGAANNSGCGAPVHDPDYIGGIGKELIVDDASDVTSFYPSAFQEHLNFIPAPTTGSGCTRIPSFDISATHYCYTHNVILSGCRDHSHSHQYLALGVLRTSATGRVFFSTLRSINLDDNQNRKSCSVSATPLGCDMLCSKITETEEEDYSSVTPTSMVHGRLGFDGQYHEKDLDVITLFKDWVANYPGVGGGSFIDNRVWFPVYGGLKPNSPSDTVQEGRYVIYKRYNDTCPDEQDYQIRMAKSSYKPGRFGGKRVQQAILSIKVSTSLGEDPVLTIPPNTVTLMGAEGRVLTVGTSHFLYQRGSSYFSPALLYPMTVNNKTATLHSPYTFNAFTRPGSVPCQASARCPNSCVTGVYTDPYPLIFHRNHTLRGVFGTMLDDGQARLNPVSAVFDNISRSRITRVSSSRTKAAYTTSTCFKVVKTNKTYVLSIAEISNTLFGEFRIVPLLVEILKNDGV

68.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.