HIFI Dna polymerase Protein, Thermococcus kodakarensis

HIFI DNA polymerase is known for its excellent processivity, rapid polymerization rate, and excellent DNA synthesis fidelity. It also has 3' to 5' exonuclease activity. Related to intein-encoded endonucleases, it suggests a potential role in intein mobility through site-specific recombination. HIFI Dna polymerase Protein, Thermococcus kodakarensis is the recombinant HIFI Dna polymerase protein, expressed by E. coli , with hFc labeled tag. HIFI Dna polymerase Protein, Thermococcus kodakarensis, has molecular weight of ~90 kDa.

HIFI DNA polymerase is characterized by its exceptional processivity, rapid polymerization rate, and remarkable fidelity in synthesizing DNA. Beyond its polymerase activity, it also possesses 3' to 5' exonuclease activity. Notably, HIFI DNA polymerase is associated with intein-encoded endonucleases, suggesting a potential role in intein mobility through site-specific recombination initiated by endonuclease cleavage at the 'homing site' in genes lacking the intein. In E. coli, PI-PkoI recognizes the minimal sequence 5'-GATTTTAGATCCCTGTACC-3' and cleaves after T-10, while PI-PkoII recognizes 5'-CAGCTACTACGGTTAC-3' and cleaves after C-10. The enzyme's efficacy, particularly in high intracellular K(+) environments, emphasizes its potential utility and activity in vivo.

Others

E. coli

hFc

P77933

3233723  [NCBI]

MASAILDTDYITEDGKPVIRIFKKENGEFKIEYDRTFEPYFYALLKDDSAIEEVKKITAERHGTVVTVKRVEKVQKKFLGRPVEVWKLYFTHPQDVPAIRDKIREHPAVIDIYEYDIPFAKRYLIDKGLVPMEGDEELKMLAFDIETLYHEGEEFAEGPILMISYADEEGARVITWKNVDLPYVDVVSTEREMIKRFLRVVKEKDPDVLITYNGDNFDFAYLKKRCEKLGINFALGRDGSEPKIQRMGDRFAVEVKGRIHFDLYPVIRRTINLPTYTLEAVYEAVFGQPKEKVYAEEITTAWETGENLERVARYSMEDAKVTYELGKEFLPMEAQLSRLVGQPLWDVSRSSTGNLVEWFLLRKAYERNEVAPNKPSEEEYQRRLRESYTGGFVKEPEKGLWENIVYLDFRALYPSIIITHNVSPDTLNLEGCKNYDIAPQVGHKFCKDIPGFIPSLLGHLLEERQKIKTKMKETQDPIEKILLDYRQKAIKLLANSFYGYYGYAKARWYCKECAESVTAWGRKYIELVWKELEEKFGFKVLYIDTDGLYATIPGGESEEIKKKALEFLKYINAKLPGALELEYEGFYKRGFFVTKKKYAVIDEEGKITTRGLEIVRRDWSEIAKETQARVLEALLKDGDVEKAVRIVKEVTEKLSKYEVPPEKLVIHEQITRDLKDYKATGPHVAVAKRLAARGVKIRPGTVISYIVLKGSGRIGDRAIPFDEFDPTKHKYDAEYYIENQVLPAVERILRAFGYRKEDLRYQKTRQVGLSAWLKPKGTLE

Approximately 90 kDa

Greater than 95% as determined by reducing SDS-PAGE.