HVCN1 Protein, Mouse (Cell-Free, His)

The HVCN1 protein plays a crucial role in cellular function by mediating voltage-dependent proton permeability, forming proton-selective channels that allow protons to pass along electrochemical gradients. This channel promotes proton efflux, which in combination with membrane depolarization contributes to the generation of reactive oxygen species during phagocytosis. HVCN1 Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived HVCN1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of HVCN1 Protein, Mouse (Cell-Free, His) is 269 a.a., with molecular weight of 36.7 kDa.

HVCN1 Protein assumes a crucial role in cellular function by mediating the voltage-dependent proton permeability of excitable membranes, forming a proton-selective channel that allows the passage of protons according to their electrochemical gradient. This channel facilitates proton efflux, coupled with membrane depolarization, contributing to the acute production of reactive oxygen species during phagocytosis. Notably, the protein forms dimers that exhibit cooperative channel gating, emphasizing the intricacy of its regulatory mechanisms. Furthermore, the channel activity is subject to inhibition by zinc ions, adding an additional layer of modulation to its functionality. HVCN1's ability to govern proton permeability and its responsiveness to membrane potential changes underscore its significance in cellular processes, particularly those associated with phagocytosis and reactive oxygen species generation.

Mouse

E. coli Cell-free

N-10*His

Q3U2S8 (M1-N269)

74096

MTSHDPKAVTRRTKVAPTKRMSRFLKHFTVVGDDYHTWNVNYKKWENEEEEEEPAPTSAEGEGNAEGPDAEAGSASTPRQSLDFRSRLRKLFSSHRFQVIIICLVVLDALLVLAELLLDLKIIEPDEQDYAVTAFHYMSFAILVFFMLEIFFKIFVFRLEFFHHKFEILDAFVVVVSFVLDLVLLFKSHHFEALGLLILLRLWRVARIINGIIISVKTRSERQILRLKQINIQLATKIQHLEFSCSEKEQEIERLNKLLKQNGLLGDVN

36.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.