ILVBL Protein, Human (Cell-Free, His)

The ILVBL protein functions as an endoplasmic reticulum 2-OH acyl-CoA lyase and plays a crucial role in the cleavage reaction (especially the removal of the C1 unit) during fatty acid α-oxidation. This enzymatic activity is performed in a thiamine pyrophosphate (TPP)-dependent manner, emphasizing the importance of cofactors involved in the catalytic process. ILVBL Protein, Human (Cell-Free, His) is the recombinant human-derived ILVBL protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of ILVBL Protein, Human (Cell-Free, His) is 632 a.a., with molecular weight of 70.7 kDa.

ILVBL protein serves as an endoplasmic reticulum 2-OH acyl-CoA lyase, playing a crucial role in the cleavage (C1 removal) reaction within the fatty acid alpha-oxidation process, a reaction dependent on thiamine pyrophosphate (TPP). This enzyme is specifically involved in the degradation of phytosphingosine, contributing to the phytosphingosine degradation pathway. ILVBL's participation in fatty acid metabolism highlights its significance in cellular energy homeostasis, particularly in the breakdown of fatty acids for energy production. The TPP-dependent nature of its catalytic activity underscores the reliance on coenzyme thiamine pyrophosphate for the enzymatic process. Overall, ILVBL plays a pivotal role in lipid metabolism, specifically in the alpha-oxidation of fatty acids and the degradation of phytosphingosine. (

Human

E. coli Cell-free

N-10*His

A1L0T0 (M1-V632)

10994

METPAAAAPAGSLFPSFLLLACGTLVAALLGAAHRLGLFYQLLHKVDKASVRHGGENVAAVLRAHGVRFIFTLVGGHISPLLVACEKLGIRVVDTRHEVTAVFAADAMARLSGTVGVAAVTAGPGLTNTVTAVKNAQMAQSPILLLGGAASTLLQNRGALQAVDQLSLFRPLCKFCVSVRRVRDIVPTLRAAMAAAQSGTPGPVFVELPVDVLYPYFMVQKEMVPAKPPKGLVGRVVSWYLENYLANLFAGAWEPQPEGPLPLDIPQASPQQVQRCVEILSRAKRPLMVLGSQALLTPTSADKLRAAVETLGVPCFLGGMARGLLGRNHPLHIRENRSAALKKADVIVLAGTVCDFRLSYGRVLSHSSKIIIVNRNREEMLLNSDIFWKPQEAVQGDVGSFVLKLVEGLQGQTWAPDWVEELREADRQKEQTFREKAAMPVAQHLNPVQVLQLVEETLPDNSILVVDGGDFVGTAAHLVQPRGPLRWLDPGAFGTLGVGAGFALGAKLCRPDAEVWCLFGDGAFGYSLIEFDTFVRHKIPVMALVGNDAGWTQISREQVPSLGSNVACGLAYTDYHKAAMGLGARGLLLSRENEDQVVKVLHDAQQQCRDGHPVVVNILIGRTDFRDGSIAV

70.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.