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  4. INSL4 Protein, Human (HEK293, His)

INSL4 Protein, Human (HEK293, His)

Cat. No.: HY-P70833
COA Handling Instructions

The INSL4 protein plays a potentially critical role in the regulation of trophoblast development and bone formation. The specific mechanism of trophoblast development deserves further study. INSL4 Protein, Human (HEK293, His) is the recombinant human-derived INSL4 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of INSL4 Protein, Human (HEK293, His) is 114 a.a., with molecular weight of ~9.0 kDa.

For research use only. We do not sell to patients.

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Description

The INSL4 protein plays a potentially critical role in the regulation of trophoblast development and bone formation. The specific mechanism of trophoblast development deserves further study. INSL4 Protein, Human (HEK293, His) is the recombinant human-derived INSL4 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of INSL4 Protein, Human (HEK293, His) is 114 a.a., with molecular weight of ~9.0 kDa.

Background

INSL4 Protein emerges as a potential key player, suggesting its crucial role in trophoblast development and the regulation of bone formation. The specific mechanisms through which INSL4 contributes to the intricate processes associated with trophoblast development remain to be fully elucidated, warranting further investigation into its functional significance in this context. Additionally, its potential involvement in the regulation of bone formation hints at a broader impact on skeletal development. The dual roles proposed for INSL4 underscore its significance in critical biological processes, prompting further research to unravel the precise molecular pathways and regulatory mechanisms through which INSL4 exerts its effects on trophoblast development and bone formation.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

Q14641 (A26-T139)

Gene ID
Molecular Construction
N-term
INSL4 (A26-T139)
Accession # Q14641
6*His
C-term
Synonyms
Early Placenta Insulin-Like Peptide; EPIL; Insulin-Like Peptide 4; Placentin; INSL4
AA Sequence

AELRGCGPRFGKHLLSYCPMPEKTFTTTPGGWLLESGRPKEMVSTSNNKDGQALGTTSEFIPNLSPELKKPLSEGQPSLKKIILSRKKRSGRHRFDPFCCEVICDDGTSVKLCT

Molecular Weight

Approximately 9.0 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

INSL4 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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INSL4 Protein, Human (HEK293, His)
Cat. No.:
HY-P70833
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