KCNJ16 Protein, Human (Cell-Free, His)

The KCNJ16 protein is a member of inwardly rectifying potassium channels, which facilitates the influx of potassium into cells. Extracellular potassium concentration modulates voltage dependence, resulting in a positive voltage shift. KCNJ16 Protein, Human (Cell-Free, His) is the recombinant human-derived KCNJ16 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of KCNJ16 Protein, Human (Cell-Free, His) is 418 a.a., with molecular weight of 50.8 kDa.

KCNJ16 protein, belonging to the family of inward rectifier potassium channels, exhibits a distinctive property favoring the influx of potassium into the cell over efflux. The voltage dependence of these channels is modulated by extracellular potassium concentrations, resulting in a shift toward more positive voltages with elevated external potassium levels. Internal magnesium primarily contributes to the inward rectification by impeding outward current. Notably, KCNJ16 is implicated in the regulation of fluid and pH balance. In the kidney, in conjunction with KCNJ10, it plays a crucial role in mediating basolateral K(+) recycling in distal tubules, a process essential for Na(+) reabsorption. The formation of a heterodimer with Kir4.1/KCNJ10 is integral for KCNJ16 localization to the basolateral membrane in kidney cells. Moreover, as a heterodimer with KCNJ10, KCNJ16 may engage in an interaction with MAGI1, potentially facilitating the expression of the potassium channel at the basolateral membrane in kidney cells. Additionally, there is a possibility of heterodimer formation with Kir2.1/KCNJ2.

Human

E. coli Cell-free

N-10*His

Q9NPI9 (M1-M418)

3773

MSYYGSSYHIINADAKYPGYPPEHIIAEKRRARRRLLHKDGSCNVYFKHIFGEWGSYVVDIFTTLVDTKWRHMFVIFSLSYILSWLIFGSVFWLIAFHHGDLLNDPDITPCVDNVHSFTGAFLFSLETQTTIGYGYRCVTEECSVAVLMVILQSILSCIINTFIIGAALAKMATARKRAQTIRFSYFALIGMRDGKLCLMWRIGDFRPNHVVEGTVRAQLLRYTEDSEGRMTMAFKDLKLVNDQIILVTPVTIVHEIDHESPLYALDRKAVAKDNFEILVTFIYTGDSTGTSHQSRSSYVPREILWGHRFNDVLEVKRKYYKVNCLQFEGSVEVYAPFCSAKQLDWKDQQLHIEKAPPVRESCTSDTKARRRSFSAVAIVSSCENPEETTTSATHEYRETPYQKALLTLNRISVESQM

50.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.