KCNJ16 Protein, Rat (Cell-Free, His)

KCNJ16 is a member of the inwardly rectifying potassium channel, which facilitates potassium influx and is regulated by extracellular potassium concentration. Its inward rectification involves the suppression of outward current by internal magnesium. KCNJ16 Protein, Rat (Cell-Free, His) is the recombinant rat-derived KCNJ16 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of KCNJ16 Protein, Rat (Cell-Free, His) is 419 a.a., with molecular weight of 54.0 kDa.

KCNJ16, a member of inward rectifier potassium channels, exhibits a preference for allowing potassium influx rather than efflux, with its voltage sensitivity modulated by extracellular potassium concentration. The channel's inward rectification is primarily attributed to the inhibition of outward current by internal magnesium. Functionally, KCNJ16 is implicated in the regulation of fluid and pH balance. Particularly in the kidney, KCNJ16, in conjunction with KCNJ10, facilitates basolateral K(+) recycling in distal tubules, a critical process for Na(+) reabsorption in these tubules. As a heterodimer with KCNJ10, KCNJ16 forms an essential interaction with MAGI1, potentially contributing to the expression of potassium channels at the basolateral membrane in kidney cells. Moreover, KCNJ16 may engage in heterodimerization with Kir2.1/KCNJ2, expanding its functional associations within cellular membranes.

Rat

E. coli Cell-free

N-10*His

P52191 (M1-M419)

/

MSYYGSSYRIVNVDSKYPGYPPEHAIAEKRRARRRLLHKDGSCNVYFKHIFGEWGSYMVDIFTTLVDTKWRHMFVVFSLSYILSWLIFGSIFWLIALHHGDLLSDPDITPCVDNVHSFTAAFLFSLETQTTIGYGYRCVTEECSVAVLTVILQSILSCIINTFIIGAALAKMATARKRAQTIRFSYFALIGMRDGKLCLMWRIGDFRPNHVVEGTVRAQLLRYSEDSEGRMTMAFKDLKLVNDQIILVTPVTIVHEIDHESPLYALDRKAVAKDNFEILVTFIYTGDSTGTSHQSRSSYVPREILWGHRFHDVLEVKRKYYKVNCLQFEGSVEVYAPFCSAKQLDWKDQQLNNLEKTSPARGSCTSDTNTRRRSFSAVAMVSSCENPEETSLSPQDECKEVPYQKALLTLNRISMESQM

54.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.