KCNN4 Protein, Human (Cell-Free, His)

KCNN4 is a voltage-dependent potassium channel that is activated by intracellular calcium, leading to membrane hyperpolarization and subsequent calcium influx. This channel is critical for the reactivation of naive T cells, helping to maximize calcium influx and promote T cell proliferation. KCNN4 Protein, Human (Cell-Free, His) is the recombinant human-derived KCNN4 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of KCNN4 Protein, Human (Cell-Free, His) is 427 a.a., with molecular weight of 59.1 kDa.

The KCNN4 (Potassium Intermediate/Small Conductance Calcium-Activated Channel, Subfamily N, Member 4) protein functions as a voltage-independent potassium channel activated by intracellular calcium, leading to membrane hyperpolarization and subsequent promotion of calcium influx. It is essential for maximal calcium influx and proliferation during the reactivation of naive T-cells, highlighting its significance in immune response modulation. Additionally, KCNN4 plays a role in the late stages of EGF-induced macropinocytosis, underscoring its involvement in cellular processes beyond ion channel activity. Notably, the channel is inhibited by clotrimazole and charybdotoxin but remains insensitive to apamin, providing insight into potential pharmacological interventions for modulating KCNN4 function.

Human

E. coli Cell-free

N-10*His

O15554 (M1-K427)

/

MGGDLVLGLGALRRRKRLLEQEKSLAGWALVLAGTGIGLMVLHAEMLWFGGCSWALYLFLVKCTISISTFLLLCLIVAFHAKEVQLFMTDNGLRDWRVALTGRQAAQIVLELVVCGLHPAPVRGPPCVQDLGAPLTSPQPWPGFLGQGEALLSLAMLLRLYLVPRAVLLRSGVLLNASYRSIGALNQVRFRHWFVAKLYMNTHPGRLLLGLTLGLWLTTAWVLSVAERQAVNATGHLSDTLWLIPITFLTIGYGDVVPGTMWGKIVCLCTGVMGVCCTALLVAVVARKLEFNKAEKHVHNFMMDIQYTKEMKESAARVLQEAWMFYKHTRRKESHAARRHQRKLLAAINAFRQVRLKHRKLREQVNSMVDISKMHMILYDLQQNLSSSHRALEKQIDTLAGKLDALTELLSTALGPRQLPEPSQQSK

59.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.