LCLAT1 Protein, Human (Cell-Free, His)

The LCLAT1 protein is an acyl-CoA:lysocardiolipin acyltransferase (ALCAT) that crucially catalyzes the reacylation of lyscardiolipin to cardiolipin (CL) during remodeling. It shows substrate flexibility, preferring linoleyl-CoA and oleoyl-CoA as acyl donors for mono- and di-cardiolipins. LCLAT1 Protein, Human (Cell-Free, His) is the recombinant human-derived LCLAT1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of LCLAT1 Protein, Human (Cell-Free, His) is 414 a.a., with molecular weight of 55.0 kDa.

LCLAT1 protein functions as an acyl-CoA:lysocardiolipin acyltransferase (ALCAT), playing a crucial role in catalyzing the reacylation of lyso-cardiolipin to cardiolipin (CL), a pivotal step in CL remodeling. This enzyme exhibits substrate flexibility, recognizing both monolysocardiolipin and dilysocardiolipin, with a preference for linoleoyl-CoA and oleoyl-CoA as acyl donors. Additionally, LCLAT1 demonstrates 1-acyl-sn-glycerol-3-phosphate acyltransferase activity (AGPAT), converting 1-acyl-sn-glycerol-3-phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3-phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone. Furthermore, it possesses lysophosphatidylinositol acyltransferase (LPIAT) and lysophosphatidylglycerol acyltransferase (LPGAT) activities. Beyond its enzymatic functions, LCLAT1 is essential for the establishment of hematopoietic and endothelial lineages, highlighting its critical role in developmental processes.

Human

E. coli Cell-free

N-10*His

Q6UWP7 (M1-E414)

253558

MHSRGREIVVLLNPWSINEAVSSYCTYFIKQDSKSFGIMVSWKGIYFILTLFWGSFFGSIFMLSPFLPLMFVNPSWYRWINNRLVATWLTLPVALLETMFGVKVIITGDAFVPGERSVIIMNHRTRMDWMFLWNCLMRYSYLRLEKICLKASLKGVPGFGWAMQAAAYIFIHRKWKDDKSHFEDMIDYFCDIHEPLQLLIFPEGTDLTENSKSRSNAFAEKNGLQKYEYVLHPRTTGFTFVVDRLREGKNLDAVHDITVAYPHNIPQSEKHLLQGDFPREIHFHVHRYPIDTLPTSKEDLQLWCHKRWEEKEERLRSFYQGEKNFYFTGQSVIPPCKSELRVLVVKLLSILYWTLFSPAMCLLIYLYSLVKWYFIITIVIFVLQERIFGGLEIIELACYRLLHKQPHLNSKKNE

55.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.