M6PR Protein, Bovine (Cell-Free, His)

M6PR (mannose 6-phosphate receptor) transports phosphorylated lysosomal enzymes from the Golgi complex to lysosomes. Lysosomal enzymes with mannose phosphate residues specifically bind to Golgi M6PR. M6PR Protein, Bovine (Cell-Free, His) is the recombinant bovine-derived M6PR protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of M6PR Protein, Bovine (Cell-Free, His) is 251 a.a., with molecular weight of 30.8 kDa.

The M6PR (Mannose-6-Phosphate Receptor) facilitates the transport of phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes carrying phosphomannosyl residues exhibit specific binding to mannose-6-phosphate receptors within the Golgi apparatus. The formed receptor-ligand complex is subsequently transported to an acidic prelysosomal compartment, where the low pH facilitates the dissociation of the complex. The M6PR functions as a homodimer and interacts with GGA1, GGA2, and GGA3, contributing to the orchestration of the cellular machinery involved in the targeted delivery of lysosomal enzymes for proper lysosomal function.

Bovine

E. coli Cell-free

N-10*His

P11456 (T29-M279)

281291

TEEKTCDLVGEKGKESEKELALLKRLTPLFNKSFESTVGQSPDMYSYVFRVCREAGNHSSGAGLVQINKSNGKETVVGRFNETQIFNGSNWIMLIYKGGDEYDNHCGREQRRAVVMISCNRHTLADNFNPVSEERGKVQDCFYLFEMDSSLACSPEISHLSVGSILLVTLASLVAVYIIGGFLYQRLVVGAKGMEQFPHLAFWQDLGNLVADGCDFVCRSKPRNVPAAYRGVGDDQLGEESEERDDHLLPM

30.8 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.