MARCHF5 Protein, Mouse (Cell-Free, His)

MARCHF5 protein is a mitochondrial E3 ubiquitin protein ligase that actively regulates mitochondrial fission and affects mitochondrial morphology. Additionally, it plays a key role in preventing cellular senescence and aids in mitochondrial quality control. MARCHF5 Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived MARCHF5 protein, expressed by E. coli Cell-free , with N-6*His labeled tag. The total length of MARCHF5 Protein, Mouse (Cell-Free, His) is 278 a.a., with molecular weight of 32.2 kDa.

MARCHF5 protein serves as a mitochondrial E3 ubiquitin-protein ligase, exerting significant influence over mitochondrial morphology by positively regulating mitochondrial fission. Beyond its role in shaping mitochondrial structure, MARCHF5 is implicated in the prevention of cell senescence, functioning as a key player in mitochondrial quality control. Its activity involves the ubiquitination of critical proteins involved in mitochondrial dynamics, namely FIS1, DNM1L, and MFN1. Through these regulatory actions, MARCHF5 emerges as a multifaceted contributor to the maintenance of mitochondrial integrity and cellular homeostasis.

Mouse

E. coli Cell-free

N-6*His

Q3KNM2 (M1-A278)

/

MPDQALQQMLDRSCWVCFATDEDDRTAEWVRPCRCRGSTKWVHQACLQRWVDEKQRGNSTARVACPQCNAEYLIVFPKLGPVVYVLDLADRLISKACPFAAAGIMVGSIYWTAVTYGAVTVMQVVGHKEGLDVMERADPLFLLIGLPTIPVMLILGKMIRWEDYVLRLWRKYSNKLQILNSIFPGIGCPVPRIPAEANPLADHVSATRILCGALVFPTIATIVGKLMFSSVNSNLQRTILGGIAFVAIKGAFKVYFKQQQYLRQAHRKILNYPEQEEA

32.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.