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  4. MCHB Protein, E.coli (Cell-Free, His)

MCHB Protein, E.coli (Cell-Free, His)

Cat. No.: HY-P702373
Handling Instructions

MCHB Protein, a bactericidal antibiotic, exhibits antimicrobial activity against bacteria closely related to its producing strain. MCHB Protein, E.coli (Cell-Free, His) is the recombinant E. coli-derived MCHB protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of MCHB Protein, E.coli (Cell-Free, His) is 60 a.a., with molecular weight of 10.9 kDa.

For research use only. We do not sell to patients.

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Description

MCHB Protein, a bactericidal antibiotic, exhibits antimicrobial activity against bacteria closely related to its producing strain. MCHB Protein, E.coli (Cell-Free, His) is the recombinant E. coli-derived MCHB protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of MCHB Protein, E.coli (Cell-Free, His) is 60 a.a., with molecular weight of 10.9 kDa.
Background

MCHB, a bactericidal antibiotic, demonstrates antimicrobial activity against bacteria that are phylogenetically related to the producing strain.
Species

E.coli
Source

E. coli Cell-free
Tag

N-10*His
Accession

P62530 (G16-S75)
Gene ID

/
Molecular Construction
N-term
10*His
MCHB (G16-S75)
Accession # P62530
C-term
Synonyms
Microcin H47; MccH47
AA Sequence

GGAPATSANAAGAAAIVGALAGIPGGPLGVVVGAVSAGLTTAIGSTVGSGSASSSAGGGS

Molecular Weight

10.9 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

MCHB Protein, E.coli (Cell-Free, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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