MCU Protein, Mouse (Cell-Free, His)

MCU protein is a mitochondrial inner membrane calcium uniporter that forms the pore of the uniplex to promote calcium uptake by mitochondria. MICU1 and MICU2 complexly regulate their activity, with low Ca(2+) levels down-regulating and high Ca(2+) levels stimulating MCU. MCU Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived MCU protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of MCU Protein, Mouse (Cell-Free, His) is 301 a.a., with molecular weight of 37.7 kDa.

MCU protein, a mitochondrial inner membrane calcium uniporter, serves as the pore-forming and calcium-conducting subunit of the uniporter complex (uniplex), mediating the uptake of calcium into mitochondria. Its activity is intricately regulated by MICU1 and MICU2, with low Ca(2+) levels down-regulating MCU activity and higher Ca(2+) levels stimulating MCU activity. This regulatory mechanism plays a crucial role in mitochondrial calcium homeostasis, influencing cellular physiology, bioenergetics, and various cellular signaling pathways. In cardiomyocytes, MCU participates in buffering systolic calcium rises, contributing to cardiac function. Additionally, MCU plays a key role in acute stress responses, facilitating a rapid increase in mitochondrial calcium in pacemaker cells. It is involved in processes such as mitochondrial permeability transition during ischemia-reperfusion injury, regulation of glucose-dependent insulin secretion in pancreatic beta-cells, modulation of muscle size in adults, and influencing synaptic vesicle endocytosis kinetics in central nerve terminals. The activity of MCU is inhibited by compounds like ruthenium red or its derivative Ru360.

Mouse

E. coli Cell-free

N-10*His

Q3UMR5 (A50-E350)

215999

AHQRPASWQSVGAAYCSTVVPSDDVTVVYQNGLPVISVRLPSRRERCQFTLKPISDSVGVFLRQLQEEDRGIDRVAIYSPDGVRVAASTGIDLLLLDDFKLVINDLTYHVRPPKRDLLSHEDAATLNDVKTLVQQLYTTLCIEQHQLNKERELVERLEDLKQQLAPLEKVRIEISRKAEKRTTLVLWGGLAYMATQFGILARLTWWEYSWDIMEPVTYFITYGSAMAMYAYFVMTRQEYVYPEARDRQYLLFFHKGAKKSRFDLEKYNQLKDAIAQAEMDLKRLRDPLQVHLPLRQIGEKE

37.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.