OTOP1 Protein, Mouse (Cell-Free, His)

The OTOP1 protein is a proton-selective channel that transports protons into less voltage-sensitive cells. This uniqueness is critical for cell types because changes in intracellular pH regulate biochemical and developmental processes. OTOP1 Protein, Mouse (Cell-Free, His) is the recombinant mouse-derived OTOP1 protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of OTOP1 Protein, Mouse (Cell-Free, His) is 600 a.a., with molecular weight of 68.6 kDa.

The OTOP1 protein functions as a proton-selective channel with a specific role in transporting protons into cells. Notably, its proton channel activity displays weak sensitivity to voltage. This distinctive characteristic suggests that the protein is particularly crucial in cell types where changes in intracellular pH serve as signaling cues or regulatory elements for biochemical and developmental processes. In the inner ear's vestibular system, OTOP1 is indispensable for the formation and function of otoconia—calcium carbonate crystals responsible for sensing gravity and acceleration. Its role likely involves maintaining the pH conducive to otoconia formation. Additionally, OTOP1 plays a regulatory role in purinergic control of intracellular calcium in vestibular supporting cells and is implicated in sour taste perception by mediating proton entry within the cytosol of sour taste cells. Furthermore, OTOP1 contributes to energy metabolism, mitigating adipose tissue inflammation and offering protection against obesity-induced metabolic dysfunction.

Mouse

E. coli Cell-free

N-10*His

Q80VM9 (M1-I600)

21906

MPGGPGAPSSPAASSGSSRAAPSGIAACPLSPPPLARGSPQASGPRRGASVPQKLAETLSSQYGLNVFVAGLLFLLAWAVHATGVGKSDLLCVLTALMLLQLLWMLWYVGRSYMQRRLIRPKDTHAGARWLRGSITLFAFITVVLGCLKVAYFIGFSECLSATEGVFPVTHAVHTLLQVYFLWGHAKDIIMSFKTLERFGVIHSVFTNLLLWANSVLNESKHQLNEHKERLITLGFGNITIVLDDHTPQCNCTPPALCSALSHGIYYLYPFNIEYQILASTMLYVLWKNIGRRVDSSQHQKMQCRFDGVLVGSVLGLTVLAATIAVVVVYMIHIGRSKSKSESALIMFYLYAITVLLLMGAAGLVGSWIYRVDEKSLDESKNPARKLDVDLLVATGSGSWLLSWGSILAIACAETRPPYTWYNLPYSVLVIVEKYVQNIFIIESVHLEPEGVPEDVRTLRVVTVCSSEAAALAASTLGSQGMAQDGSPAVNGNLCLQQRCGKEDQESGWEGATGTTRCLDFLQGGMKRRLLRNITAFLFLCNISLWIPPAFGCRPEYDNGLEEIVFGFEPWIIVVNLAMPFSIFYRMHAAAALFEVYCKI

68.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.