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  4. Resistin Protein, Mouse (HEK293, Fc)

Resistin Protein, Mouse (HEK293, Fc)

Cat. No.: HY-P73680
SDS COA Handling Instructions

Resistin hormone inhibits insulin's efficacy in promoting glucose uptake into adipose cells, potentially linking obesity and diabetes. Structurally, it forms stabilizing disulfide linkages as a homodimer. The interplay between resistin and insulin reveals complex molecular mechanisms in metabolic regulation, providing insights into how resistin may contribute to the pathophysiological connections between obesity and diabetes. Resistin Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Resistin protein, expressed by HEK293 , with N-hFc labeled tag. The total length of Resistin Protein, Mouse (HEK293, Fc) is 94 a.a., with molecular weight of ~39 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

Resistin hormone inhibits insulin's efficacy in promoting glucose uptake into adipose cells, potentially linking obesity and diabetes. Structurally, it forms stabilizing disulfide linkages as a homodimer. The interplay between resistin and insulin reveals complex molecular mechanisms in metabolic regulation, providing insights into how resistin may contribute to the pathophysiological connections between obesity and diabetes. Resistin Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Resistin protein, expressed by HEK293 , with N-hFc labeled tag. The total length of Resistin Protein, Mouse (HEK293, Fc) is 94 a.a., with molecular weight of ~39 kDa.

Background

Resistin, an influential hormone, appears to play a pivotal role in inhibiting insulin's efficacy in promoting glucose uptake into adipose cells, thereby establishing a potential association between obesity and diabetes. Structurally, it adopts a homodimeric form, characterized by stabilizing disulfide linkages. The intricate interplay between resistin and insulin underscores the complex molecular mechanisms involved in metabolic regulation, offering valuable insights into how resistin's actions may contribute to the pathophysiological connections between obesity and the development of diabetes.

Biological Activity

Measured in a cell proliferation assay using PC-3 cells. The ED50 for this effect is 32.38 ng/mL, corresponding to a specific activity is 3.09×10^4 units/mg.

  • Measured in a cell proliferation assay using PC-3 cells. The ED50 for this effect is 32.38 ng/mL, corresponding to a specific activity is 3.09×104 units/mg.
Species

Mouse

Source

HEK293

Tag

N-hFc

Accession

Q99P87 (S21-S114)

Gene ID
Molecular Construction
N-term
hFc
Resistin (S21-S114)
Accession # Q99P87
C-term
Synonyms
Resistin; Adipose tissue-specific secretory factor; Cysteine-rich secreted protein FIZZ3; FIZZ3; ADSF; RSTN; RETN
AA Sequence

SSMPLCPIDEAIDKKIKQDFNSLFPNAIKNIGLNCWTVSSRGKLASCPEGTAVLSCSCGSACGSWDIREEKVCHCQCARIDWTAARCCKLQVAS

Molecular Weight

Approximately 39 kDa

Purity
  • Greater than 90% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Resistin Protein, Mouse (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Resistin Protein, Mouse (HEK293, Fc)
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HY-P73680
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