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  4. RHD Protein, Human (Cell-Free, His)

RHD Protein, Human (Cell-Free, His)

Cat. No.: HY-P702423
Handling Instructions

RHD Protein is a member of the ammonium transporter (TC 2.A.49) family, particularly within the Rh subfamily. RHD Protein, Human (Cell-Free, His) is the recombinant human-derived RHD protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of RHD Protein, Human (Cell-Free, His) is 417 a.a., with molecular weight of 51.2 kDa.

For research use only. We do not sell to patients.

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Description

RHD Protein is a member of the ammonium transporter (TC 2.A.49) family, particularly within the Rh subfamily. RHD Protein, Human (Cell-Free, His) is the recombinant human-derived RHD protein, expressed by E. coli Cell-free , with N-10*His labeled tag. The total length of RHD Protein, Human (Cell-Free, His) is 417 a.a., with molecular weight of 51.2 kDa.
Background

RHD Protein belongs to the ammonium transporter (TC 2.A.49) family, specifically within the Rh subfamily.
Species

Human
Source

E. coli Cell-free
Tag

N-10*His
Accession

Q7RU08 (M1-F417)
Gene ID

/
Molecular Construction
N-term
10*His
RHD (M1-F417)
Accession # Q7RU08
C-term
Synonyms
RHD; Rh blood group; Rh blood group D antigen; Rh blood group, D antigen; RhD polypeptide; RhD protein; Rhesus blood group D antigen; Rhesus blood group D antigen variant; Rhesus blood group, D antigen
AA Sequence

MSSKYPRSVRRCLPLWALTLEAALILLFYFFTHYDASLEDQKGLVASYQVGQDLTVMAAIGLGFLTSSFRRHSWSSVAFNLFMLALGVQWAILLDGFLSQFPSGKVVITLFSIRLATMSALSVLISVDAVLGKVNLAQLVVMVLVEVTALGNLRMVISNIFNTDYHMNMMHIYVFAAYFGLSVAWCLPKPLPEGTEDKDQTATIPSLSAMLGALFLWMFWPSFNSALLRSPIERKNAVFNTYYAVAVSVVTAISGSSLAHPQGKISKTYVHSAVLAGGVAVGTSCHLIPSPWLAMVLGLVAGLISVGGAKYLPGCCNRVLGIPHSSIMGYNFSLLGLLGEIIYIVLLVLDTVGAGNGMIGFQVLLSIGELSLAIVIALTSGLLTGLLLNLKIWKAPHEAKYFDDQVFWKFPHLAVGF

Molecular Weight

51.2 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

RHD Protein, Human (Cell-Free, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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